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上海浩然生物技术有限公司

品名:DIG-High Prime DNA Labeling and Detection Starter Kit II （DNA高效地高辛标记及检测试剂盒II ）
检测方法:Kit for chemiluminescent detection with CSPD'' ready-to use.
Catalog :11585614910
Pack size :1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay'' blots of 100 cm2
Contents
1.DIG-High Prime'' 5x conc.'' 50 µl
2.DIG-labeled Control DNA'' 20 µl'' (5 µg/ml) pBR328 (linearized with Bam HI)
3.DNA Dilution Buffer'' 3 x 1 ml
4.Anti-digoxigenin-AP Conjugate'' 50 µl
5.CSPD'' ready-to-use'' 50 ml
6.Blocking Solution'' 10x conc.'' 4 x 100 ml
7.DIG Easy Hyb Granules'' 4 x 100 ml
Application
DIG-High Prime labeled probes can be used in northern'' Southern-'' and dot-blot analysis'' as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP'' alkali-labile'' and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind'' offering ready-to-use CSPD supplied with a dripping device for easy application'' ready-made blocking solution'' and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme'' nucleotides'' primers'' and reaction buffer'' all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency'' plasmid'' cosmid'' and phage DNA'' linearized or supercoiled can be used as templates'' yielding the same labeling efficiency.
Product Description
Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling'' and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization'' 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot'' loading 1 µg of digested placenta DNA.