神经探针AA96，AB96和AC96趋化性室使用框架式过滤器，粘附细胞可在其上染色并在显微镜下计数或在自动荧光读数器中计数。A系列96孔趋化室由可重复使用的精密加工丙烯酸顶板和底板，装配硬件和硅胶垫圈组成。底板有96个孔，顶板上相应的孔形成96个上部孔。框架式过滤器将上井和下井分开。
在孵育期间，添加到下部孔中的化学引诱物作用于添加到上部孔中的悬浮液中的细胞。细胞通过过滤器迁移，并计算过滤器上迁移的贴壁细胞。
框架式过滤器由粘合到刚性框架上的过滤膜组成，无需镊子即可方便地进行处理。过滤器准确地卡入腔室中，并且在孵育之后，过滤器可以通过其框架保持，同时擦拭，染色并准备计数细胞。框架的外部尺寸和过滤器上96个位置的格式设计为适合标准荧光读数器，因此荧光标记的贴壁细胞可以自动计数。
顶板有六个定位销，确保轻松准确的装配。硅树脂垫圈打孔有对应于定位销的孔，并在组装之前安装在顶板上。带框架的过滤器紧贴销钉。当垫片夹在顶板和底板之间时，垫圈形成密封。除硬件外，组装腔室的整体尺寸为5.75“x 5.00”x .90“。
三个A系列腔室的容积和暴露的过滤面积不同，如下：
| 股票 ＃ | 暴露过滤面积（mm2） | 井径（mm） | 上井容量
（μL） | 较低的井容量
（μL） |
|---|
| AA96 | 32 | 6.4 | 390 | 80 |
|---|
| AB96 | 18 | 4.8 | 225 | 三十 |
|---|
| AC96 | 8 | 3.2 | 二百二十五分之五十* | 25 |
|---|
* AC96在上部井中有一个台阶; 如果填充到台阶，容量为50μl，如果填充到顶部则为225μl。
伴随每个腔室的是 A系列96孔协议， 用于其使用和护理。有关 正确使用的信息，请参阅 框架式过滤器。 可提供96孔腔室过滤器 和 更换垫圈 。
Neuro Probe AA96, AB96, and AC96 chemotaxis chambers use framed filters on which adherent cells can be stained and counted under a microscope or counted in an automated fluorescence reader. The A-Series 96-well chemotaxis chambers consist of reusable precision-machined acrylic top and bottom plates, assembly hardware, and a silicone gasket. The bottom plate has 96 wells, and corresponding holes in the top plate form the 96 upper wells. The framed filter separates the upper and lower wells.
During incubation chemoattractants added to the lower wells act on cells in a suspension added to the upper wells. Cells migrate through the filter, and migrated adherent cells on the filter are counted.
The framed filter consists of a filter membrane bonded to a rigid frame that can be conveniently handled without forceps. The filter snaps accurately into the chamber, and after incubation the filter can be held by its frame while wiping, staining, and preparing to count cells. The external dimensions of the frame and the format of the 96 sites on the filter are designed to fit standard fluorescence readers, so fluorescent-tagged adherent cells can be automatically counted.
The top plate has six registration pins to ensure easy and accurate assembly. The silicone gasket is punched with holes corresponding to the registration pins and is installed on the top plate prior to assembly. The framed filter fits snugly against the pins. The gasket forms a seal when sandwiched with the filter between the top and bottom plates. Overall dimensions of the assembled chamber, excluding hardware, is 5.75″ x 5.00″ x .90″.
The three A-series chambers differ in well volume and exposed filter area, as follows:
| Stock # | Exposed Filter Area (mm2) | Well diameter (mm) | Upper Well Capacity
(μL) | Lower Well Capacity
(μL) |
|---|
| AA96 | 32 | 6.4 | 390 | 80 |
|---|
| AB96 | 18 | 4.8 | 225 | 30 |
|---|
| AC96 | 8 | 3.2 | 50/225* | 25 |
|---|
* The AC96 has a step in the upper well; capacity is 50μl if filled to the step, 225μl if filled to the top.
Accompanying each chamber is an A-series 96-well protocol for its use and care. See framed filters for information on their proper use. 96-well chamber filters and replacement gaskets are available.