V01001必拓生物pET21a(+)载体，pET21a(+) vector，质粒载体图谱
C-末端His6标签
成分：10 mM Tris（pH8.0）
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01001 | pET21a(+) vector | 10μl，20ng/μl | 500
|
pET21a(+)载体，pET21a(+) vector，质粒载体图谱
LOCUS pET-21a(+) 5443 bp DNA circular 23-JUL-2013
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
COMMENT ORIGDB|GenBank
COMMENT VNTDBDATE|668342873|
COMMENT LSOWNER|
COMMENT VNTNAME|pET-21a(+)|
FEATURES Location/Qualifiers
misc_feature 946..1803
/vntifkey="21"
/label=bla(Amp)
misc_feature 359..814
/vntifkey="21"
/label=f1\origin
promoter 21..37
/vntifkey="30"
/label=T7\promoter
misc_feature 40..64
/vntifkey="21"
/label=lac\operator
terminator 276..322
/vntifkey="43"
/label=T7\terminator
misc_feature 106..190
/vntifkey="21"
/label=MCS
misc_feature 191..208
/vntifkey="21"
/label=His6
misc_feature complement(3998..5077)
/vntifkey="21"
/label=lacI
misc_feature 109..141
/vntifkey="21"
/label=T7\tag
rep_origin 1867..3034
/vntifkey="33"
/label=ColE1\origin
BASE COUNT 1268 a 1441 c 1446 g 1288 t
ORIGIN
1 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
61 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat ggctagcatg
121 actggtggac agcaaatggg tcgcggatcc gaattcgagc tccgtcgaca agcttgcggc
181 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
241 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
301 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
361 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
421 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
481 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
541 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
601 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
661 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
721 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
781 gcgaatttta acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg
841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
1021 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
1081 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
1141 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
1201 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
1261 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
1321 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
1381 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
1441 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
1501 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
1561 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
1621 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
1681 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
1741 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
1801 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
1861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
2101 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
2641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
2701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
2761 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
2821 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
2881 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
2941 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
3001 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
3061 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
3121 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
3181 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
3241 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
3301 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
3361 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
3421 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
3481 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
3541 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
3601 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
3661 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
3721 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
3781 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
3841 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
3901 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
3961 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
4021 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
4081 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
4141 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
4201 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
4261 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
4321 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
4381 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
4441 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
4501 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
4561 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
4621 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
4681 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
4741 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
4801 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
4861 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
4921 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
4981 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
5041 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
5101 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
5161 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
5221 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
5281 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
5341 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
5401 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcg
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