| Catalogue Number | CYT-308 |
| Synonyms | Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF. |
| Introduction | Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. MCSF induces cells of the monocyte/macrophage lineage. MCSF plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. |
| Description | Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD.
MCSF is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The MCSF protein was lyophilized with 10mM sodium Phosphate, pH-8.0 & 50mM NaCl. |
| Solubility | It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids of MCSF was determined and found to be Met-Glu-Glu-Val-Ser. |
| Purity | Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. |
| Biological Activity | The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found to be < 2.0ng/ml, corresponding to a specific activity of 500,00Units/mg. |
| References | 1.Title:Osteoclasts Control Osteoblast Chemotaxis via PDGF-BB/PDGF Receptor Beta Signaling.
Publication:Sanchez-Fernandez MA, Gallois A, Riedl T, Jurdic P, Hoflack B (2008) Osteoclasts Control Osteoblast Chemotaxis via PDGF-BB/PDGF Receptor Beta Signaling. PLoS ONE 3(10): e3537. doi:10.1371/journal.pone.0003537.
Link:http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0003537#references
2.Title:Suppressor of cytokine signalling-3 at pathological levels does not regulate lipopolysaccharide or interleukin-10 control of tumour necrosis factor-? production by human monocytes.
Publication:Article first published online: 11 MAY 2006 DOI: 10.1111/j.1365-2567.2006.02383.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2567.2006.02383.x/full
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| Usage | ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |