The E.coli derived recombinant 58kDa protein is a genetically engineered peptide which is derived from Dengue Type-1 and 3 to be expressed as a fused envelope, it was designed specifically for gold conjugation in a rapid test production, it has proved better results in specificity and sensitivity compared to individual single antigen by a rapid test company located at San Diego, USA. This fusion protein is connected to a 6xHis Tag. Dengue Type-1 and 3 is purified by proprietary chromatographic technique.