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| 英文名称 |
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| 国产/进口 |
进口 |
| 产地/品牌 |
OROBOROS/奥地利 |
| 型号 |
Oxygraph-2k |
| 参考报价 |
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| 销售商 |
世联博研北京科技有限公司 |
Oxygraph-2k线粒体呼吸测定系统 |
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Oxygraph-2k高分辨线粒体呼吸测定系统
OROBOROS Oxygraph-2k是高分辨率的呼吸测定仪。相对于市场上的传统呼吸测定产品，这套系统具有效率高，分辨率高的特别。这款仪器为生物能学，线粒体生理学，线粒体病理的临床诊断和研究树立了一个新的标准。
OROBOROS Oxygraph-2k 用于一些低呼吸活性,快速代谢反应和低氧水平的呼吸测定,在这些领域高分辨率的测定仪器是需要的。
? 病理现像导致的呼吸微弱（细胞凋亡，线粒体或新陈代射疾病，老化，离体-再灌注损伤，过氧应激）
? 活检取样，样品量比较少（尢其是那些诊断遗传疾病和线粒体疾病的儿科病人）
? 量比较少的细胞培养
? 突变导致呼吸能力下降
? 氧化率和抗氧化能力检测（质量控制）
? 低水平吸吸检测，生理细胞内氧水平或氧活性
Oxygraph-2k技术参数
主机参数
尺寸（主机） L45cm,W31cm,H25cm
净重 20.5kg
内建电子控温的Peltier温度控制单元
温度范围 4-45℃
温度稳定性 0.005℃，90分钟之后
温度变化速度 20-30℃，15分钟
30-20℃，20分钟
RS232数据输出接口
? 两个仓内的氧信号
? 压力信号，吸收的压力信号，分辨率0.1kPa
? 温度信号，分辨率0.001℃
DatLab软件
? 同步显示流动氧的浓度
? 自动进行氧信号的校正
? 多传感器信号采集
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O2k-Manual
The O2k-Manual is an open-access guide to the use of the Oxygraph-2k and DatLab, TIP2k and O2k-MultiSensor Modules.
Contents O2k-Manual
Contents O2k-Manual. MiPNet17.11. - download pdf
User Information
User information. MiPNet07.08. - download pdf
O2k-Start: High-Resolution Respirometry - O2k-Core A
Oxygraph-2k: Start high-resolution respirometry. MiPNet12.06.
Updated for O2k-Series E-F and DatLab 5.1.
OroboPOS Service - O2k-Core B
Service of the polarographic oxygen sensor OroboPOS. MiPNet08.04.
DatLab Guide - O2k-Core C
DatLab guide through the menus. MiPNet12.07.
Updated for DatLab 5.1.
O2k-Calibration - O2k-Core D
Oxygen and pX (pH) calibration by DatLab. MiPNet12.08.
Updated for DatLab 5.1.
Oxygen Flux Analysis - O2k-Core E
Oxygen flux analysis: DatLab on-line. MiPNet12.09.
Updated for DatLab 5.1.
TIP2k-Manual
Titration-Injection microPump TIP2k. User manual. MiPNet12.10.
O2k-Fluorescence LED2-Module
O2k-Fluorescence LED2-Module. MiPNet17.05.
O2k-TPP+ and Ca2+ ISE-Module
O2k-MultiSensor system with ion selective electrodes (ISE). MiPNet15.03
O2k-NO Amp-Module
O2k-MultiSensor system with amperometric electrodes. MiPNet15.05
PBI-Shredder HRR-Set
PBI-Shredder HRR-Set: Preparation of tissue homogenates.
MiPNet17.02 - (Auxiliary HRR Tool).
An application study with the PBI-Shredder. MiPNet17.15
Further links
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O2k-Protocols
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O2k-Publications
>>> O2k-Catalogue OROBOROS - a guided tour to the O2k
>>> Troubleshooting
Archive: DatLab2
OROBOROS Oxygraph-2k
- to explore the Power of Life
O2k - the modular system for
high-resolution respirometry, HRR
'OXPHOS analysis extends simple bioenergetics to the level of mitochondrial physiology' (Gnaiger 2012).
We thank all colleagues and O2k-Customers, particularly our MiPNet Reference Laboratories and participants at Bioblast 2012, for making 2012 the most successful out of 20 years of high-resolution respirometry. We thank you for your positive feedback, and wish you all the best and good luck in 2013.
The OROROROS INSTRUMENTS office is closed for Christmas Holidays until 1st January 2013. We will be available intermittently from 2nd - 4th January.
OROBOROS is back to regular hours on 7th January 2013.
Happy New Year!
The O2k-Team
O2k
- The world's most Powerful, Popular and Prestigeous respirometer for cellular and mitochondrial research.a
- High efficiency - low running costs.b
- O2k-MultiSensor: Combining HRR with fluorometry (ROS production, mt-membrane potential, ATP-production, Ca2+), potentiometric measurement of pH, TPP+ and Ca2+, amperometric measurement of NO.c
- World-wide standard for diagnosis of mitochondrial function in health and disease.
- Flexibility for respiration of isolated mitochondria, homogenates, cultured cells and small tissue biopsies.d
- Substrate-uncoupler-inhibitor titration (SUIT) protocols with multiple respiratory states in a single experimental incubation: no other platform is suited for application of SUIT protocols.
- Flexibility within experiments, on-line display of concentration and flux, automatic software controlled titrations.e
Reference:
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
- a new Quality in Science
O2k-Feedback: 'Once you've had a taste of high-performance, there's no going back'.
get O2k-Connected .. it's not Warburg, it's O2k
OROBOROS - Top 10 Reasons: Leading technology, sole source, long-term experience, economical, no investors, no expensive advertising, scientific committment, Corporate Social Responsibility ...
OROBOROS MiPArt
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Gentle Science Shapes the World
Mitochondrial Physiology Society - MiP www.mitophysiology.org
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?Bioblast wiki ?O2k-Catalogue ?O2k-Fluorescence ?MitoPedia Glossary: Respiratory states
Footnotes
(a) 888 O2k-Publications; O2k-WorldWide
HRR since 1992 - Ref: Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.
(b) High efficiency - low running costs
(c) O2k-Fluorescence; O2k-TPP and Ca2+; O2k-pH; O2k-NO
(d) HRR of isolated mitochondria, intact cells, permeabilized cells, permeabilized tissues
(e) TIP2k
Ref: Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.