非酒精性脂肪性肝炎(NASH)…

非酒精性脂肪性肝炎(NASH)建模----专题二NASH动物模型(HFD法)
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  • 参考报价:电议 产地:美国 品牌:MPBIO 型号:D12451-01 更新时间:2012/8/24

 

专题二 NASH动物模型(HFD法)

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除了MCD法,HFD(high-fat diet)法用于诱导动物脂肪变性和NASH的应用也十分普遍。因为与MCD法诱导的NASH动物模型相比,HFD诱导的NASH模型在发病机制上更相似于人NASH。经诱导的啮齿类动物呈现出体重增加,体内脂肪水平提高以及胰岛素抵抗等特征。HFD法诱导大鼠NASH模型的结果最为可信。研究显示,Sprague-Dawley大鼠喂养6周后患肥胖症,表现出ALT异常、脂肪肝、炎症、高脂血症、高胰岛素血症、高血糖症及IR等体征,另外喂养3周后动物体重和血浆ALT水平不会提高。总而言之,HFD诱导的NASH动物模型在脂肪变性、炎症和纤维化几个方面表现出个体上的差异,主要受动物种类、饲料中脂肪成分、饲料组成和喂养时间相关。

Research Diets公司提供两种类型的HFD动物饲料(45% kcal% fat.),分别有粉末状(powder)和颗粒状(pellet)。另外还提供LFD对照饲料(10% kcal% fat),方便客户监控造模的成功性及观察动物模型体内相关指标的变化。

? 高脂饲料(HFD)配方

HFD fomulationtotal4.73 kcal/gm

LFD fomulationtotal3.85 kcal/gm

成分

重量(gm

能量(kcal

成分

重量(gm

能量(kcal

Casein, 80 Mesh

200

800

Casein, 80 Mesh

200

800

L-Cystine

3

12

L-Cystine

3

12

Corn Starch

72.8

291

Corn Starch

315

1260

Maltodextrin

100

400

Maltodextrin 10

35

140

Sucrose

172.8

691

Sucrose

350

1400

Cellulose, BW200

50

0

Cellulose, BW200

50

0

Soybean Oil

25

225

Soybean Oil

25

225

Lard*

177.5

1598

Lard*

20

180

Mineral Mix S10026

10

0

Mineral Mix S10026

10

0

DiCalcium Phosphate

13

0

DiCalcium Phosphate

13

0

Calcium Carbonate

5.5

0

Calcium Carbonate

5.5

0

Potassium Citrate,

1 H2O

16.5

0

Potassium Citrate,

1 H2O

16.5

0

Vitamin Mix V10001

10

40

Vitamin Mix V10001

10

40

Choline Bitartrate

2

0

Choline Bitartrate

2

0

FD&C Red Dye #40

0.05

0

FD&C Red Dye #40

0.05

0

Total

858.15

4057

Total

1055.05

4057

*Typical analysis of cholesterol in lard = 0.95 mg/gram.

Cholesterol (mg)/4057 kcal = 168.6

Cholesterol (mg)/kg = 196.5

*Typical analysis of cholesterol in lard = 0.95 mg/gram.

Cholesterol (mg)/4057 kcal= 19

Cholesterol (mg)/kg = 18

? 高脂饲料(HFD)订购信息

产品名称

货号

规格

产品形式

目录价

HFD(45% kcal% fat)

D12451-01

12.5 kg

Pellet

Inquiry

D12451-02

12.5 kg

Powder

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LFD10% kcal% fat

D12450B-01

12.5 kg

Pellet

Inquiry

D12450B-02

12.5 kg

Powder

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? 看看您需要pellet还是powder形式的产品?

Pellets

Powder

Pellets are easier to feed than powder. Pellets can simply be thrown on top of the cage or even inside. Its easy to monitor the pellet supply and assure that animals have 24/7 access to feed.

Powder requires special feeders. The feeders can be easily tipped and spilled, and the food can also be easily soiled by urine, feces or even saliva. So it is difficult to assure that the animals have 24/7 access to good clean food.

Pellets are a stable food stuff. They can be stored for months without losing quality.

Powder has much greater surface area and the food spoils much easier. Powder also has a less obvious problem in as much as powders can become ‘unmixed’ over time. That is, smaller, more dense particles may sift to the bottom and less dense, fluffier parts may sift up, making the diet no longer homogenous and in the extreme nutritionally incomplete from top or bottom.

Pellets are homogeneous.

Powder can be separated by the animal by pawing, sniffing and snorting, either intentionally or inadvertently.

Once pelleted compounds are ‘frozen’ in place and won’t become unmixed. Also the test compound is more stable in pellets as most of it is inside the pellet and away from direct air, light and humidity which cause most of the degradation of test compounds.

Powder is often used by facilities that lack the equipment, personnel, money or interest in pelleting food. This is often done when researchers want to add test compounds to feed. Test compounds can easily sift out of the powder.

Pellets are dustless and cannot be inhaled.

Powder can be (or is) inhaled by feeding animals causing respiratory irritation and infections. This is an often overlooked problem with feeding powder diets. This is especially likely with powders of standard chows, which unless irradiated are loaded with microbes.

Feeding pellets helps ‘wear’ incisors down.

Powder fed rodents may develop overgrown incisors

 

 

 

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