| 17-5056-01 |
HITRAP CM FF, 5 X 1 ML |
| 17-5079-01 |
HITRAP RPROTEIN A FF, 5X1ML |
| 17-5079-02 |
HITRAP RPROTEIN A FF, 2 X 1 ML |
| 17-5080-01 |
HITRAP RPROTEIN A FF, 1X5ML |
| 17-5080-02 |
HITRAP RPROTEIN A FF, 5 X 5 ML |
| 17-5110-01 |
HITRAP IGM PURIFICATION 5X1ML |
| 17-5111-01 |
HITRAP IGY PURIFICATION 1X5 ML |
| 17-5112-01 |
HITRAP STREPTAVIDIN HP5X1 ML |
| 17-5130-01 |
GSTRAP FF, 5X1ML |
| 17-5130-02 |
GSTRAP FF, 2X1ML |
| 17-5130-05 |
GSTRAP FF, 100 X 1 ML |
| 17-5131-01 |
GSTRAP FF, 1X5ML |
| 17-5131-02 |
GSTRAP FF 5 X 5 ML |
| 17-5131-05 |
GSTRAP FF, 100 X 5 ML |
| 17-5143-01 |
HITRAP BENZAMIDINE FF 5 X 1ML |
| 17-5143-02 |
HITRAP BENZAMIDINE FF 2 X 1 ML |
| 17-5144-01 |
HITRAP BENZAMIDINE FF 1 X 5 ML |
| 17-5154-01 |
HITRAP DEAE FF 5 X 5 ML |
| 17-5155-01 |
HITRAP CM FF, 5 X 5 ML |
| 17-5156-01 |
HITRAP Q FF, 5 X 5 ML |
| 17-5157-01 |
HITRAP SP FF, 5 X 5 ML |
| 17-5158-01 |
HITRAP Q XL, 5 X 1 ML |
| 17-5159-01 |
HITRAP Q XL, 5 X 5 ML |
| 17-5160-01 |
HITRAP SP XL, 5 X 1 ML |
| 17-5161-01 |
HITRAP SP XL, 5 X 5 ML |
HiTrap™ IEX FF Columns
- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), and ANX Sepharose™ 4 Fast Flow (high sub) (weak anion).
- Designed for use with syringe, peristaltic pump, or chromatography systems such as ÄKTA™design.
- High flow rates, and good scale-up potential.
HiTrap™ IEX FF Columns
Technical Information
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.
ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.
| TECHNICAL SPECIFICATIONS |
| Medium |
Q Sepharose™ Fast Flow |
| |
SP Sepharose™ Fast Flow |
| |
CM Sepharose™ Fast Flow |
| |
DEAE Sepharose™ Fast Flow |
| |
ANX Sepharose™ 4 Fast Flow (high sub) |
| Column volume |
1 ml or 5 ml |
| Max. flow rate* |
| 1-ml column |
4 ml/min |
| 5-ml column |
20 ml/min |
| Recommended flow rate |
| 1-ml column |
1 ml/min |
| 5-ml column |
5 ml/min |
| Max back pressure |
3 bar (43 psi, 0.3 MPa) |
| Storage |
20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature |
4°C to 30°C |
| * H2O at 25°C
|
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=164008