| TECHNICAL SPECIFICATIONS |
| Ion exchanger type |
|
| Q Sepharose™ XL |
Quaternary amino strong anion exchanger |
| SP Sepharose™ XL |
Sulfopropyl strong cation exchanger |
| Ionic capacity |
|
| Q Sepharose™ XL |
0.18-0.26 mmol Cl-/ml |
| SP Sepharose™ XL |
0.18-0.25 mmol H+/ml |
| Dynamic capacity |
|
| Q Sepharose™ XL |
> 130 mg BSA/ml medium |
| SP Sepharose™ XL |
> 160 mg lysozyme/ml medium |
| Matrix |
Dextran attached to 6% highly
cross-linked spherical agarose |
| Average particle size |
90 μm |
| pH stability |
|
| Q Sepharose™ XL |
2-14 (short term), 2-12 (long term) |
| SP Sepharose™ XL |
3-14 (short term), 4-13 (long term) |
| Chemical stability |
All commonly used buffers, nonionic
detergents, 1 M NaOH, 6 M
guanidine hydrochloride |
| Avoid using |
|
| Q Sepharose™ XL |
Oxidizing agents, anionic detergents, and buffers |
| SP Sepharose™ XL |
Oxidizing agents, cationic detergents, and buffers |
| Storage |
20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature |
4°C to 30°C |
| Prepacked HiPrep™ 16/10 Columns |
| Column volume |
20 ml |
| Column dimensions |
1.6 × 10 cm |
| Recommended flow rate* |
2-10 ml/min (60-300 cm/h) |
| Max. flow rate* |
10 ml/min (300 cm/h) |
Max. pressure over
packed bed during
operation* |
1.5 bar (22 psi, 0.15 MPa) |
Column hardware
pressure limit |
5 bar (73 psi, 0.5 MPa) |
| Storage |
20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature |
4°C to 30°C |
| * H2O at 25°C.
|