| 17-5177-01 |
MINI Q 4.6/50 PE |
| 17-5178-01 |
MINI S 4.6/50 PE |
MiniBeads™ Columns
Technical Information
MiniBeads ion exchange media are available prepacked in Tricorn and PC columns for high-performance, high-resolution purification of proteins, peptides, and polynucleotides.
| TECHNICAL SPECIFICATIONS |
| MiniBeads™ |
| Ion exchanger type |
| Mini Q™ |
Quaternary ammonium strong anion |
| Mini S™ |
Methyl sulfonate strong cation |
| Ionic capacity |
|
| Mini Q™ |
85-115 μmol Cl-/ml packed medium |
| Mini S™ |
13-29 μmol H+/ml packed medium |
| pH stability |
3-11 (working), 2-14 (short term) |
| Chemical stability |
Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. |
| Base matrix |
Monodisperse polystyrene/divinyl benzene particles |
| Particle size |
3 μm |
| Storage |
20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) |
| Storage temperature |
4°C to 30°C |
| MiniBeads™ Columns* |
| Bed volume |
|
| PC 3.2/3 |
0.24 ml |
| 4.6/50 PE |
0.8 ml |
| Max. pressure |
|
| PC 3.2/3 |
100 bar (1450 psi, 10 MPa) |
| 4.6/50 PE |
180 bar (2610 psi, 18 MPa) |
| Recommended flow rate |
|
| PC 3.2/3 |
0.1-1.0 ml/min |
| 4.6/50 PE |
0.5-2.0 ml/min |
| * Columns not suitable for use with ÄKTAprime™ plus system.
|
The exceptional resolution with Mini S 4.6/50 PE makes it a powerful tool for purity check of synthetic oligonucleotides.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165401