MabSelect™ Media and HiTrap™ MabSelect Prepacked Columns

• MabSelect media allow capture of monoclonal antibodies (Mabs) from large sample volumes.
• Prepacked HiTrap columns for screening of purification conditions and small-scale purification of Mabs.
• Media designed to process more than 10 000 l feed from high-expression fermentations in a working day at process scale.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

MabSelect™ Media and HiTrap™ MabSelect Prepacked Columns

Technical Information

MabSelect Media and HiTrap MabSelect prepacked columns for purification of monoclonal antibodies with optimized binding capacities at high flow rates

MabSelect™ SuRe LX is optimized for high dynamic binding capacity for high titer cultures of antibodies. This affinity chromatography medium is designed with the same alkali-tolerant, protein A-derived ligand as in MabSelect SuRe, making it stable in as high concentration as 0.5 M NaOH.

MabSelect™ is a BioProcess™ affinity medium for capturing Mabs from large volumes of feed by packed bed chromatography. The recombinant protein A ligand of MabSelect™ is specially engineered to favor an oriented coupling that gives an affinity medium with enhanced binding capacity for IgG. The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification. In addition, the high capacity, low ligand leakage, and the novel base matrix make MabSelect™ ideal for purification of Mabs at process scale. MabSelect™ is available prepacked in 1-ml and 5-ml HiTrap™ MabSelect™ columns for fast purification of Mabs from large sample volumes. The prepacked MabSelect™ medium is compatible with the high flow rates and high pressure required when scaling up.

MabSelect™ SuRe has been developed from the same highly cross-linked agarose matrix used for MabSelect™, which enables high flow rates at low backpressure. In contrast to the recombinant protein A ligand of MabSelect™, however, the alkali-tolerant recombinant protein A ligand of MabSelect™ SuRe is resistant to harsh cleaning agents (NaOH), which results in significant cost savings. The high alkaline tolerance of the medium also provides the possibility to extend the number of cycles in regular production.

HiTrap™ MabSelect SuRe™ columns are prepacked with MabSelect SuRe™. The high-flow matrix of MabSelect SuRe™ makes HiTrap™ MabSelect SuRe™ well suited for the purification of MAbs when scaling up. HiTrap™ MabSelect SuRe™ columns also allow simulation of cleaning-in-place in process development on account of the alkali-tolerant recombinant Protein A ligand of the prepacked medium.

MabSelect Xtra™ has been developed to meet the demands of ever-increasing levels of expression in monoclonal antibody feedstocks. MabSelect Xtra™ uses the same recombinant protein A ligand as MabSelect™, but has a smaller particle size and greater porosity, which ensures increased dynamic binding capacity. The medium provides a lower overall production cost due to the possibility of processing concentrated feedstocks in fewer batches. Process development, screening of purification conditions, and small-scale purification of Mabs can be performed using 1-ml and 5-ml HiTrap™ MabSelect Xtra™ columns.