| 17-5166-01 |
MONO Q 5/50 GL |
| 17-5167-01 |
MONO Q 10/100 GL |
| 17-5168-01 |
MONO S 5/50 GL |
| 17-5169-01 |
MONO S 10/100 GL |
| 17-5170-01 |
MONO P 5/50 GL |
| 17-5171-01 |
MONO P 5/200 GL |
| 17-5177-01 |
MINI Q 4.6/50 PE |
| 17-5178-01 |
MINI S 4.6/50 PE |
| 17-5179-01 |
MONO Q 4.6/100 PE |
| 17-5180-01 |
MONO S 4.6/100 PE |
| 17-5181-01 |
SOURCE 15Q 4.6/100 PE |
| 17-5182-01 |
SOURCE 15S 4.6/100 PE |
| 17-5186-01 |
SOURCE 15PHE 4.6/100 PE |
MonoBeads™ Columns
- Innovative, monodisperse 10-μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
- MonoBeads™ available in a choice of three prepacked column formats¾Tricorn™, HR, and PC columns.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- Compatible with ÄKTAdesign™, FPLC™ System, and other high-performance LC systems.
- MonoBeads™ are also available as Mono P for chromatofocusing
MonoBeads™ Columns
Technical Information
MonoBeads™ are prepacked in Tricorn™, HR, and PC column formats. The three Tricorn™ (5/50 GL, 10/100 GL, and 4.6/100 PE) columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads™ columns (see page 511).
Tricorn™ columns are ideal for the polishing step when high purity is required. HR 16/10 columns, on the other hand, have higher loading capacity than Tricorn™ columns. PC 1.6/5 columns are ideal for micropurification of subnanogram to microgram amounts of sample on ÄKTA Systems or other high-performance LC systems.
Customer feedback on Mono Q HR 5/5.
| TECHNICAL SPECIFICATIONS |
| MonoBeads™ |
| Ion exchanger type |
Mono Q™ |
Strong anion |
| |
Mono S™ |
Strong cation |
| Ionic capacity |
Mono Q™ |
0.27-0.37 mmol Cl-/ml |
| |
Mono S™ |
0.12-0.15 mmol H+/ml |
| pH stability |
|
2-12 (working), 1-14 (short term) |
| Chemical stability |
|
Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. |
| Medium |
|
Monodisperse polystyrene/divinyl benzene particles |
| Particle size |
|
10 μm |
| Storage |
|
20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) |
| Storage temperature |
4°C to 30°C |
| MonoBeads Columns* |
| Bed volume |
PC 1.6/5 |
0.1 ml |
| |
5/50 GL |
1 ml |
| |
4.6/100 PE |
1.7 ml |
| |
10/100 GL |
8 ml |
| |
HR 16/10 |
20 ml |
| Max. pressure |
PC 1.6/5 |
50 bar (725 psi, 5 MPa) |
| |
5/50 GL, 10/100 GL,
4.6/100 PE |
40 bar (580 psi, 4 MPa) |
| |
HR 16/10 |
30 bar (435 psi, 3 MPa) |
| Recommended flow rate |
PC 1.6/5 |
0.01-0.4 ml/min |
| |
5/50 GL, 4.6/100 PE |
0.5-3.0 ml/min |
| |
10/100 GL |
2.0-6.0 ml/min |
| |
HR 16/10 |
< 10 ml/min |
* Columns are not suitable for use with ÄKTAprime™ plus system.
|
Tricorn high-performance columns are prepacked with MonoBeads ion exchange media for high-resolution purification and high capacity.
The polishing step on Mono Q 5/50 GL shows different phosphoforms of the purified kinase and very high purity.