| 28-9365-41 |
HiPrep DEAE FF 16/10 |
| 28-9365-42 |
HiPrep CM FF 16/10 |
| 28-9365-43 |
HiPrep Q FF 16/10 |
| 28-9365-44 |
HiPrep SP FF 16/10 |
| 28-9365-45 |
HiPrep Phenyl FF (high sub) 16 |
| 28-9365-46 |
HiPrep Phenyl FF (low sub) 16/ |
| 28-9365-47 |
HiPrep Butyl FF 16/10 |
| 28-9365-48 |
HiPrep Octyl FF 16/10 |
| 28-9365-49 |
HiPrep Heparin FF 16/10 |
| 28-9365-50 |
GSTPrep FF 16/10 |
| 28-9365-51 |
HisPrep FF 16/10 |
| 28-9365-52 |
HiPrep IMAC FF 16/10 |
HiPrep™ IEX FF 16/10 Columns
- Q, SP, DEAE, and CM Sepharose™ 6 Fast Flow in prepacked 20-ml HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
- Ideal for rapid enrichments during initial capture of proteins from starting material.
- Simple operation.
- Compatible with single-pump configurations and other chromatography systems such as ÄKTA™design
HiPrep™ IEX FF 16/10 Columns
Technical Information
HiPrep™ Q FF16/10 , HiPrep™ SP FF 16/10, HiPrep™ DEAE FF 16/10 and HiPrep™ CM FF 16/10 combine good flow rates and high binding capacity.
| TECHNICAL SPECIFICATIONS |
| Media |
Q Sepharose™ Fast Flow
SP Sepharose™ Fast Flow
DEAE Sepharose™ Fast Flow
CM Sepharose™ Fast Flow |
| Column volume |
20 ml |
| Column dimensions |
1.6 × 10 cm |
| Recommended flow rate* |
2-10 ml/min (60-300 cm/h) |
| Max. flow rate* |
10 ml/min (300 cm/h) |
| Max. pressure over the packed bed during operation* |
1.5 bar (22 psi, 0.15 MPa) |
| Column hardware pressure limit |
5 bar (73 psi, 0.5 MPa) |
| Storage |
20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature |
4°C to 30°C |
| *H2O at room temperature.
|
|
Separations of standard proteins on HiPrep™ CM FF 16/10 at pH 5.0, pH 6.5, and
pH 7.5.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=164266