| 28-9644-24 |
HiScale 16/40 |
| 28-9644-41 |
HiScale 16 20BH |
| 28-9644-44 |
HiScale 50 40BH |
| 28-9644-45 |
HiScale 50 20BH |
HiScale™ Empty Columns
Technical Information
HiScale 16/40, 26/40, and 50/40 columns are supplied, complete with two adapters and Valco unions for easy connection to chromatography systems
HiScale™ columns are designed to withstand pressures of up to 20 bar (290 psi) and high flow rates, making them compatible with modern BioProcess chromatography media such as MabSelect™ and Capto™. The user-friendly design allows for an optimized process, through simplified and reproducible operation.
The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments as well as disassembly and cleaning. The measurement scale on the column makes it easier to predict and reach a predefined packing height, thereby increasing process control as well as reproducibility. Although optimized for high-pressure media, HiScale columns are fully compatible with all applications (and packing methods) currently applicable to XK columns.
HiScale columns are delivered with a column tube, outer protection tube, two adapters, PEEK tubing, and unions at both ends for easy connection to valves, pumps, and monitors.
Purification and scale-up of IgG on MabSelect SuRe™ affinity chromatography medium (above) and scale-up of GFP on Capto Q IEX chromatography medium (below). The chromatogram on the top is a normalized overlay of UV absorbance (280 nm) for HiScreen™, packed with MabSelect SuRe (blue) and HiScale 16/40 packed with the same medium (red). The second chromatogram is a normalized overlay of UV absorbance (490 nm) for HiScreen Capto Q (blue) and HiScale 16/40 packed with Capto Q (red). The figure shows the excellent reproducibility during scale-up from HiScreen to HiScale with two different chromatographic techniques.
| TECHNICAL SPECIFICATIONS |
| Column dimensions (i.d. × H) |
| HiScale 16/20 |
16 mm × 20 cm |
| HiScale 16/40 |
16 mm × 40 cm |
| HiScale 26/20 |
26 mm × 20 cm |
| HiScale 26/40 |
26 mm × 40 cm |
| HiScale 50/20 |
50 mm × 20 cm |
| HiScale 50/40 |
50 mm × 40 cm |
| Max operating pressure |
20 bar (290 psi) |
| Operating temperature |
4°C to 40°C |
| pH stability |
1-14 |
| Materials in contact with eluent |
Polypropylene, borosilicate glass, polyamide, fluoro-rubber, PEEK capillary tubing, and Tefzel™ ferrules. |
| Mesh size of net |
10 μm, 20 μm, and 80 μm* nylon |
*HiScale 50 columns only
| TECHNICAL SPECIFICATIONS - Maximum Bed Volumes and Bed Height Ranges |
| Column |
Max. bed volume (ml) |
Bed height range (cm) |
| HiScale 16/20 |
40 |
0-20 |
| HiScale 16/40 |
80 |
8-40 |
| HiScale 26/20 |
106 |
0-20 |
| HiScale 26/40 |
212 |
13-40 |
| HiScale 50/20 |
393 |
0-20 |
| HiScale 50/40 |
785 |
14-40 |
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