| 17-0716-01 |
HITRAP NHS-ACTIV HP, 5 X 1ML |
| 17-0716-30 |
HiTrap NHS-activated HP, 180 x |
| 17-0717-01 |
HITRAP NHS-ACTIV HP, 1 X 5 ML |
| 17-0920-03 |
HITRAP IMAC HP, 5 X 1 ML |
| 17-0920-05 |
HITRAP IMAC HP, 5 X 5 ML |
| 17-0921-02 |
HITRAP IMAC FF, 5 X 1ML |
| 17-0921-04 |
HITRAP IMAC FF, 5 X 5 ML |
| 17-0978-13 |
HITRAP BUTYL-S FF, 5 X 1ML |
| 17-0978-14 |
HITRAP BUTYL-S FF, 5 X 5ML |
| 17-1128-01 |
MABTRAP KIT |
| 17-1151-01 |
HITRAP SP HP, 5 X 1 ML |
| 17-1152-01 |
HITRAP SP HP, 5X5 ML |
| 17-1153-01 |
HITRAP Q HP, 5 X 1 ML |
| 17-1154-01 |
HITRAP Q HP 5X5 ML |
| 17-1351-01 |
HITRAP PHENYL HP, 5 X 1ML |
| 17-1353-01 |
HITRAP PHENYL FF (LS), 5X1ML |
| 17-1355-01 |
HITRAP PHENYL FF (HS),5 X 1ML |
| 17-1357-01 |
HITRAP BUTYL FF, 5 X 1 ML |
| 17-1359-01 |
HITRAP OCTYL FF, 5 X 1 ML |
| 17-1408-01 |
HITRAP DESALTING, 5X5ML |
| 17-5053-01 |
HITRAP Q FF, 5 X 1 ML |
| 17-5054-01 |
HITRAP SP FF, 5 X 1 ML |
| 17-5055-01 |
HITRAP DEAE FF 5 X 1 ML |
| 17-5056-01 |
HITRAP CM FF, 5 X 1 ML |
HiTrap™ NHS-activated HP Columns
- Prepacked with NHS-activated Sepharose™ High Performance for coupling ligand via primary amines at near neutral pH.
- Simple coupling, carried out at close to neutral pH.
- Delivered in 100% isopropanol to protect the active groups.
HiTrap™ NHS-activated HP Columns
Technical Information
Lane 1: Desorbed material, reduced
Lane 2: Low Molecular Weight (LMW) calibration kit, reduced
Lane 1: Desorbed material, non-reduced
Lane 2: Low Molecular Weight (LMW) calibration kit, reduced
The use of NHS-activated Sepharose™ High Performance is illustrated by the purification of anti-mouse Fc-IgG from sheep antiserum after coupling mouse IgG to HiTrap™ NHS-activated. The contents of the different fractions were analysed by SDS-PAGE using PhastSystem™ and Goomassie staining.
| TECHNICAL SPECIFICATIONS |
| Group to be coupled |
-NH2 |
| Coupling conditions |
pH 6.5-9 at 4°C to 25°C |
| Matrix |
Highly cross-linked agarose, 6% |
| Substitution |
10 μmol NHS/ml medium |
| Spacer arm |
6-aminocaproic acid, 10-atom |
| pH stability* |
3-12 |
| Average particle size |
34 μm |
| Storage |
100% isopropanol |
| Storage temperature |
4°C to 8°C |
| * Ligand dependent.
|
For column specifications, see Prepacked HiTrap™ Affinity Columns.
Purification of anti-mouse Fc-IgG from sheep antiserum.