| 28-9365-50 |
GSTPrep FF 16/10 |
1 ×20 ml |
GSTPrep™ FF 16/10 Column - Easy Scale-up Purification
Technical Information
GSTPrep™ FF 16/10 for fast and easy scale-up purification of GST-tagged recombinant proteins.
GSTPrep™ FF 16/10 are 20-ml HiPrep™ columns prepacked with Glutathione Sepharose™ 4 Fast Flow for scale-up purification of recombinant GST-tagged proteins, other glutathione S-transferases, and glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow in combination with HiPrep™ column hardware provides excellent flow properties.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165074
| TECHNICAL SPECIFICATIONS |
| Medium |
Glutathione Sepharose™ 4 Fast Flow |
| Column volume |
20 ml |
| Column dimensions |
1.6 × 10 cm |
| Recommended flow rate* |
1-10 ml/min (30-300 cm/h) |
| Max. flow rate* |
10 ml/min (300 cm/h) |
| Max. pressure over the packed bed during operation* |
1.5 bar (0.15 MPa, 22 psi) |
| Column hardware pressure limit |
5 bar (0.5 MPa, 73 psi) |
| Storage |
20% ethanol |
| Storage temperature |
4°C to 30°C |
* H2O at room temperature.
Note: Binding of GST to glutathione is flow-rate dependent and lower flow rates
often increase the binding capacity. This is important to consider, especially
during sample loading and elution. |