| 17-5172-01 |
SUPEROSE 6 10/300 GL |
| 17-5173-01 |
SUPEROSE 12 10/300 GL |
| 17-5174-01 |
SUPERDEX 75 10/300 GL |
| 17-5175-01 |
SUPERDEX 200 10/300 GL |
| 17-5176-01 |
SUPERDEX PEPTIDE 10/300 GL |
| 28-9065-61 |
Superdex 200 5_150 GL |
| 28-9205-04 |
Superdex 75 5_150 GL |
| 17-0506-01 |
PP COLUMN MONO Q HR 16/10 |
| 17-0507-01 |
PP COLUMN MONO S HR 16/10 |
| 17-1002-01 |
MONO Q 60/100 |
| 17-1177-01 |
RESOURCE Q, 1 ML |
| 17-1178-01 |
RESOURCE S, 1 ML |
| 17-1179-01 |
RESOURCE Q, 6 ML |
| 17-1180-01 |
RESOURCE S, 6 ML |
| 17-1184-01 |
RESOURCE ETH 1 ML |
| 17-1185-01 |
RESOURCE ISO 1 ML |
| 17-1186-01 |
RESOURCE PHE 1 ML |
| 17-1187-01 |
RESOURCE HIC TEST KIT |
| 17-5166-01 |
MONO Q 5/50 GL |
| 17-5167-01 |
MONO Q 10/100 GL |
| 17-5168-01 |
MONO S 5/50 GL |
| 17-5169-01 |
MONO S 10/100 GL |
| 17-5170-01 |
MONO P 5/50 GL |
| 17-5171-01 |
MONO P 5/200 GL |
| 17-5177-01 |
MINI Q 4.6/50 PE |
| 17-5178-01 |
MINI S 4.6/50 PE |
| 17-5179-01 |
MONO Q 4.6/100 PE |
| 17-5180-01 |
MONO S 4.6/100 PE |
| 17-5181-01 |
SOURCE 15Q 4.6/100 PE |
| 17-5182-01 |
SOURCE 15S 4.6/100 PE |
| 17-5186-01 |
SOURCE 15PHE 4.6/100 PE |
| 18-4271-00 |
O RING 64.5X3 EPDM GLASS TUBE |
| 18-4284-01 |
FILTER TOOL, 60 MM |
| 18-4610-60 |
DISTRIBUTION UNIT 60 MM TI 1 |
| 18-4610-62 |
O RING 55X1.6 EPDM |
Superdex™ 75 / Superdex™ 200 Columns
- High-resolution separations of proteins, peptides, and other biomolecules according to size.
- Ideal for the polishing step in a purification procedure.
- Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns
Superdex™ 75 / Superdex™ 200 Columns
Technical Information
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn™ Column Family, that provide excellent reproducibility and durability in purification using a chromatography system.
| TECHNICAL SPECIFICATIONS |
| Superdex™ |
| Exclusion limit (Mr) |
| Superdex™ 75 |
1 × 105 globular protein |
| Superdex™ 200 |
1.3 × 106 globular protein |
| Separation range (Mr) |
| Superdex™ 75 |
3000-70 000 globular protein |
| Superdex™ 200 |
10 000-600 000 globular protein |
| Matrix |
Spherical composite of cross-linked agarose and dextran |
| Average particle size |
13 μm |
| Chemical stability |
Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning in place) |
| pH stability |
3-12 (working and long term), 1-14 (short term) |
| Storage |
20% ethanol |
| Storage temperature |
4°C to 30°C |
| Superdex™ 10/300 GL Columns (Tricorn)* |
| Bed dimensions |
10 × 300 mm |
| Recommended sample volume |
25-250 μl |
| Bed volume |
24 ml |
| Max. pressure |
|
| Superdex™ 75 |
18 bar (261 psi, 1.8 MPa) |
| Superdex™ 200 |
15 bar (217 psi, 1.5 MPa) |
| Max. flow rate (H2O at 25°C) |
| Superdex™ 75 |
1.5 ml/min |
| Superdex™ 200 |
1.0 ml/min |
| Theoretical plates |
> 30 000 m-1 |
| Superdex™ PC 3.2/30 Columns |
| Bed dimensions |
3.2 × 300 mm |
| Bed volume |
2.4 ml |
| Recommended sample volume |
2-25 μl |
| Max. pressure |
|
| Superdex™ 75 |
24 bar (348 psi, 2.4 MPa) |
| Superdex™ 200 |
12 bar (174 psi, 1.2 MPa) |
| Max. flow rate (H2O at 25°C) |
| Superdex™ 75 |
0.100 ml/min |
| Superdex™ 200 |
0.100 ml/min |
| Theoretical Plates |
> 30 000 m-1 |
| Superdex™ 5/150 column (Tricorn™) |
| Bed volume |
3 ml |
| Bed dimensions |
5 × 150 mm |
| Recommended sample volume |
4 - 50 μl |
| Recommended flow rate |
0.15 - 0.6 ml/min |
| Max. pressure |
|
| Superdex 75 |
1.8 MPa |
| Superdex 200 |
1.5 MPa |
| Max. flow rate (H20 at 25°C) |
|
| Superdex 75 |
0.7 ml/min |
| Superdex 200 |
0.8 ml/min |
| Theoretical plates |
> 25000 m-1 |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
|
Separation of standard proteins on Tricorn™ Superdex™ 200.
Separation of monomer and dimer on Tricorn™ Superdex™ 75.
Column performance test with a set of standard proteins on Superdex™ 75
5/150 GL.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165422