GLUTATHIONESEPH4FF

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GLUTATHIONESEPH4FF
产品简介
详细介绍
  • 参考报价：电议 产地：美国 品牌：GE 型号：17-5132-02 更新时间：2017/3/23

17-5132-02 GLUTATHIONE SEPH 4 FF, 100 ML
17-5132-03 GLUTATHIONE SEPH 4 FF, 500 ML
17-5138-01 RMP PROT A SEPH FF 5 ML
17-5138-02 RMP PROT A SEPH FF 25 ML
17-5138-03 RMP PROT A SEP FF 200 ML
17-5199-01 MABSELECT 25 ML
17-5199-02 MABSELECT 200 ML
17-5268-01 NI SEPHAROSE HP 25 ML
17-5268-02 NI SEPHAROSE HP 100 ML
17-5269-02 MABSELECT XTRA 200 ML
17-5269-07 MABSELECT XTRA 25ML
17-5279-01 GLUTATHIONE SEPHAROSE HP 25 ML
17-5279-02 GLUTATHIONE SEPH HP 100 ML
17-5280-01 NPROTEIN A SEPHAROSE 4 FF 5ML
17-5280-02 NPROTEIN A SEPH 4 FF 200 ML
17-5280-04 NPROTEIN A SEPHAROSE 4FF 25ML
17-5318-01 NI SEPHAROSE 6FF, 25 ML
17-5318-02 NI SEPHAROSE 6FF, 100 ML
17-5318-03 NI SEPHAROSE 6FF, 500 ML
17-5318-06 NI SEPHAROSE 6 FF 5 ML
17-5438-01 MABSELECT SURE 25 ML
17-5438-02 MABSELECT SURE 200 ML
17-5448-01 Capto Blue 25 ml
17-5474-01 MabSelect SuRe LX 25 ML
17-5474-02 MabSelect SuRe LX 200 ML
17-6002-35 IMMUNOPRECIP STARTER PACK
18-1022-29 HB AFFINITY CHROMATOGRAPHY
18-1037-46 ANTIBODY PURIFICATION HB
28-9355-97 Dextrin Sepharose High Perform
28-9355-98 Dextrin Sepharose High Perform
28-9356-00 StrepTactin Sepharose High Per
28-9805-82 MembraneProteinPurificationKit
17-5468-15 HiScreen Capto SP ImpRes
17-5470-15 HiScreen Capto Q ImpRes
17-5474-15 HiScreen MabSelect SuRe LX

Glutathione Sepharose 4 Fast Flow - Scale-up Purification

  • Fast, one-step purification of GST*-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins.
  • Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm.
  • Excellent for scale-up due to high capacity and high flow properties.

Technical Information


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Glutathone Sepharose 6 Fast Flow for purification of GST-tagged proteins.

 

Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.

For more information of the full range of products included in the Glutathione S-transferase (GST*) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System. A more comprehensive guide to the GST Gene Fusion System is available in handbook 18-1157-58, see Media Reference Books, Software and Video Cassettes.

 

TECHNICAL SPECIFICATIONS
Ligand  Glutathione 
Ligand coupling method  Epoxy activation 
Matrix  Highly cross-linked agarose, 4% 
Average particle size  90 μm 
Binding capacity*  » 10 mg recombinant GST/ml medium 
pH stability  3-12 
Chemical stability  All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH,  70% ethanol, 6 M guanidine hydrochloride (1 h at room temperature) 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
*Binding of GST to glutathione is flow-rate dependent and lower flow rates often
increase the binding capacity. This is important to consider during sample
loading and elution.

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