bio-rad 161-0737 Laemmli样品缓冲液 30ml
Introduction
Bio-Rad’s Laemmli sample buffer is based
on the method of Laemmli1 (1970). The use
of Laemmli sample buffer ensures optimal
band resolution when preparing proteins for
SDS-PAGE with Tris-glycine-SDS running
buffer.
Specifications
Composition 62.5 mM Tris-HCl, pH 6.8
25% glycerol
2% SDS
0.01% Bromophenol Blue
Storage Ambient temperature
Shelf life 1 year
Instructions for Use
1. Add Reducing Agent
Add 50 μl of β-mercaptoethanol per 950 μl of
sample buffer for a final concentration of 5%
β-mercaptoethanol, 710 mM. As an alternative,
dithiothreitol (DTT or Cleland’s reagent) may be
used at a final concentration of 350 mM
(54 mg/ml).
Note: For best results, do not store sample
buffer with β-mercaptoethanol.
2. Dilute Sample
Dilute 1 part sample with 1 part Laemmli
sample buffer. More sample buffer can be
added if necessary. A 1 part sample to 2 parts
sample buffer dilution also works. Dry samples
can be dissolved directly into the sample buffer.
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