产品介绍
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL12A in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 2 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120μL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
SENSITIVITY
The minimum detectable dose of IL12A is typically less than 11.9pg/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of IL12A.
No significant cross-reactivity or interference between IL12A and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between IL12A and all analogues, therefore, cross reactivity may still exist.
PRECISION
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL12A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL12A were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
STABILITY
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
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