产品介绍
ASK1 Polyclonal Antibody | ASK1 A0283.pdf |
Catalog No: A0283 | Species: Rabbit | |
Gene ID: 4217 | Isotype: IgG | |
Swiss Prot: Q99683 | Purity: Affinity purification | |
Applications: WB | Cross-Reactivity: H M R | |
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| Size | Price (USD) | Availability | | | |
| 100ug | $220 | Ship in 5 business days | Adding to the cart | | |
| 200ug | $380 | Ship in 5 business days | Adding to the cart | | |
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Alt. Names
MAP3K5;ASK1;MAPKKK5;MEKK5 ;
Recommended Dilutions
WB: 1:500-1:2000
Products data
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Western blot analysis of extracts of K562 cells, using ASK1 antibody. | | |
Immunogen
Recombinant protein of human ASK1
Storage
Store at -20oC or -80oC. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
Apoptosis signal-regulating kinase 1 (ASK1), a MAP kinase kinase kinase, plays essential roles in stress-induced apoptosis (1,2). ASK1 is activated in response to a variety of stress-related stimuli through distinct mechanisms and activates MKK4 and MKK3, which in turn activate JNK and p38 (3). Overexpression of ASK1 activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation and cell death (4,5). Phosphorylation at Ser967 is essential for ASK1 association with 14-3-3 protein and suppression of cell death (6). Oxidative stress induces dephosphorylation of Ser967 and phosphorylation of Thr845 in the activation loop of ASK1, and both are correlated with ASK1 activity and ASK1-dependent apoptosis (7,8). On the other hand, Akt phosphorylates ASK1 at Ser83, which attenuates ASK1 activity and promotes cell survival (9).
1. Ichijo, H. et al. (1997) Science 275, 90-94.
2. Wang, X.S. et al. (1996) J. Biol. Chem. 271, 31607-31611.
3. Matsuzawa, A. and Ichijo, H. (2001) J. Biochem. (Tokyo) 130, 1-8.
4. Tobiume, K. et al. (2001) EMBO Rep. 2, 222-228.
5. Nishitoh, H. et al. (2002) Genes Dev. 16, 1345-1355.
6. Zhang, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8511-8515.
7. Tobiume, K. et al. (2002) J. Cell. Physiol. 191, 95-104.
8. Goldman, E.H. et al. (2004) J. Biol. Chem. in press, .
9. Kim, A.H. et al. (2001) Mol. Cell. Biol. 21, 893-901.