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产品介绍

Alt. Names

ATG7;APG7-LIKE;APG7L;DKFZp434N0735;GSA7 ;

Recommended Dilutions

WB: 1:500-1:2000

IHC: 1:50-1:200

Products data

Immunogen

Recombinant protein of human ATG7

Storage

Store at -20oC or -80oC. Avoid freeze / thaw cycles.

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

1.     Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.

2.     Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.

3.     Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.

4.     Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.

5.     Mizushima, N. et al. (1998) Nature 395, 395-8.

6.     Suzuki, K. et al. (2001) EMBO J 20, 5971-81.

7.     Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.

8.     Shintani, T. et al. (1999) EMBO J 18, 5234-41.