性能描述
storage temp. -20°C
Amino Acid Sequence
N-Suc-Ala-Ala-Pro-Phe-pNA
Substrates
Colorimetric substrate for chymotrypsin, human leukocyte cathepsin G, and peptidyl prolyl isomerase.
Storage/Stability
Suc-AAPF-pNA has a shelf life of 3 years when stored desiccated at –20 °C.
Preparation Instructions
Suc-AAPF-pNA is soluble in N,N-dimethylformamide (DMF) at 25 mg/ml producing a clear, light yellow solution. It is also soluble in 5% dimethylformamide(v/v) in buffer10 and in distilled water at 4 mg/ml.
A 20 mM solution in dimethyl sulfoxide (DMSO) has been prepared and stored as a stock solution at 4 °C.Stock solutions in DMSO were diluted in 0.10 M HEPES buffer, pH 7.5, for the assay of cathepsin G.6
Suc-AAPF-pNA is soluble at 10 mM in 0.2 M Tris-HCl buffer, pH 8.0. In this buffer, a 1 mM solution of the substrate spontaneously hydrolyzes at a rate of about 0.1% per day at 4 °C.It is recommended to prepare aqueous solutions of Suc-AAPF-pNA immediately prior to use. Aqueous
solutions of the substrate were kept on ice during experiments.
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