UltraClean?Endotoxin-Free Mini Plasmid Prep Kit在质粒提取过程中结合了内毒素去除步骤。无需使用免疫亲和柱去除脂多糖。采用改进的碱性裂解试剂裂解细胞。内毒素选择性地沉淀下来。质粒DNA吸附到离心柱滤膜上。经过冲洗，再次释放到不含无内毒素水（试剂盒提供）中。此时质粒DNA有足够浓度直接进行下游实验。
Billaud, J.N., Peterson, D., Barr, M., Chen, A., Sallberg, M., Garduno,F., Goldstein, P., McDowell, W., Hughes, J., Jones, J., and Milich, D. CombinatorialApproach to Hepadnavirus-Like Particle Vaccine Design . Journal of Virology. 13656-13666 (2005).
Krinks, J. MicrobialAssesment the Lindesn Bioremediation System Treating Acid Mine Drainage in theHuff Run Watershed .
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