来宝网 2013/8/13点击1499次
pSicoR pGK puro载体
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VMA0373 | pSicoR pGK puro | Addgene | 慢病毒载体 |
Backbone size （bp）: 7700
Modifications to Backbone: Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5’: HpaI
Cloning site 3’: XhoI
5’ sequencing primer: mU6-F
pSicoR PGK Puro allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author’s map for pSico and on the Jacks Lab
