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pCRE-Luc

The Agilent PathDetect in Vivo Signal Transduction Pathway cis-Reporting

Systems are designed for simple, rapid, and convenient assessment of the in

vivo activation of signal transduction pathways. We have created a series of

inducible reporter plasmids that contain the luciferase reporter gene driven

by a basic promoter element （TATA box） plus a defined inducible cisenhancer element （see Figure 2 in Appendix）. These plasmids are

particularly suited for the in vivo readouts of signal transduction pathways

since these enhancers are convergent points of many signal transduction

pathways. These systems are useful in studying the in vivo effects of a new

gene, growth factor, or drug candidate on the signaling pathway. Some of

the reporters may also be used for cloning novel signal transduction genes,

and may be useful for identifying drug candidates in a high-throughput

format.

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