载体特点：与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。

pGBKT7-53 encodes a fusion of the murine p53 protein （a.a. 72–390） and the GAL4 DNA-BD （a.a. 1–147）. The murine p53 cDNA （GenBank Accession #K01700） was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. （1993）; plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.