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Luciferase-based siRNA Target Test Kit is designed to provide a quantitative approach for evaluation of sequence potential to serve as an efficient target for RNA interference (RNAi). Current computer-based RNAi target searching algorithms are not perfect, leaving the probability of selected sequence to be a good target from 30% to 60%. It is therefore clear that the ability of computer-selected sequence to induce RNA interference has to be confirmed experimentally. The siRNA Target Test Kit allows for screening a large number of potential siRNA target sequences in simple transient transfection experiments, measuring the reduction in reporter (firefly Luciferase from Photinus pyralis) gene expression.