来宝网 2015/5/22点击2554次
中国聚乙二醇修饰在蛋白药物领域的研究（PEG药物研究）
药物的聚乙二醇修饰即聚乙二醇化,是将活化的聚乙二醇通过化学方法偶联到蛋白、多肽、小分子有机药物和脂质体上是一种蛋白质工程分子修饰方法。药物的聚乙二醇修饰可分为两个阶段。
第一阶段的修饰技术局限于应用相对分子质量低的单甲氧基聚乙二醇(<20000)。常用的修饰剂有单甲氧基聚乙二醇琥珀酸琥珀酰亚胺酯(mPEG-SS)、单甲氧基聚乙二醇碳酸琥珀酰亚胺酯(mPEG-SC)等,通过酯键或三嗪环将聚乙二醇与药物分子偶联,这种非特异性的不稳定连接方式使得一个药物分子经常连接数个聚乙二醇分子。但第一代聚乙二醇修饰药物通常表现出不稳定性、较大的毒性和免疫原性,生物活性、药代动力学的性质与原型药物没有本质的改变。
以应用相对分子质量高(>20000)聚乙二醇修饰剂为特征的第二阶段的修饰技术具有连接稳定、定点修饰、控释等特点,修饰后的药物具有更高的生物活性、更好的物理及热稳定性、更高的产品均一性和纯度。
但总得说来,蛋白药物PEG修饰技术中最大的问题就是无法实现定点修饰,修饰产物不均一,给分离纯化带来很大难度,也很大程度上阻碍了临床应用。根据蛋白质的氨基酸性质和PEG衍生物的特点,科研人员开发了多种定点修饰的策略和方法。
有一些蛋白的N端对其生物活性起着重要作用,如果在N端实施PEG修饰则会使蛋白的生物活性丧失,因此将PEG修饰的位点转移至C端则是一种有效的修饰策略。一般选用mPEG-酰肼或mPEG-胺对蛋白质进行修饰,羧基的修饰位点包括天冬氨酸、谷氨酸及末端羧基。mPEG-酰肼是近年来开发的又一种可与羧基特异性结合的修饰剂。
与赖氨酸相比,半胱氨酸在蛋白质中出现几率和数量很少,利用具有巯基反应活性PEG衍生物就可以实现蛋白定点修饰,但缺点是半胱氨酸的巯基通常处于蛋。
白的活性中心,修饰后会使蛋白活性损失较大。对于没有半胱氨酸的蛋白则可以通过基因工程手段引入巯基反应位点。这一方法不但能够实现高度选择性修饰,而且能够减少蛋白生物活性的丧失,并降低免疫原性。 目前可用于巯基修饰的mPEGs包括mPEG-马来酰亚胺、mPEG-邻吡啶-二硫醚、mPEG-乙烯基砜及mPEG-碘乙酰胺等。其中mPEG-马来酰亚胺制备简单,应用较多。PEG-邻吡啶-二硫醚与蛋白质偶联形成的S—S键,在生理条件下可缓慢水解,释放出天然蛋白质,有利于活性的恢复,但修饰率低。PEG-乙烯基砜在pH7-9范围内可选择性的与巯基反应,当pH较高时则与氨基交联。PEG-碘乙酰胺的优势在于经强酸水解后,可释放出半胱氨酸衍生物,易于鉴定。
对蛋白中含有的二硫键进行PEG定点修饰分为2个步骤:首先利用温和的还原剂使二硫键还原释放两个硫氢基;第二步通过PEG修饰剂的双烷基化反应重建二硫键,从而完成PEG的定点修饰。在修饰蛋白过程中不会出现蛋白的不可逆变性,而且二硫键还原后蛋白仍维持其三级结构,PEG选择性的修饰两个硫基,PEG的立体屏障作用确保1个二硫键只结合1分子PEG。但是二硫键PEG修饰可能会由于PEG的屏蔽作用,降低蛋白的生物活性。Veronese等利用变性剂盐酸胍(3mol/L)使蛋白部分变性,高级结构打开,但不使用还原剂以保护二硫键,在非折叠状态下完成PEG修饰后,通过透析或凝胶过滤色谱的方法去除变性剂,使蛋白重新正确折叠恢复生物活性
通常只有具有极性的氨基酸残基的侧链基团才能够进行PEG化学修饰。Deiters等开发了一种定点修饰非极性氨基酸苯丙氨酸的方法,首先用叠氮化物通过亲核取代反应修饰蛋白质中的苯丙氨酸生成对位叠氮化苯丙氨酸,再用炔烃衍生的PEG-酰胺修饰剂修饰苯丙氨酸,发生环化加成反应形成稳定的连接体,从而完成对蛋白的定点修饰。另外,通过基因重组在蛋白质中引入含叠氮基团的氨基酸残基,再与特定PEG修饰剂偶联形成稳定的定点单修饰产物。Cazalis通过基因工程方法在血栓调节蛋白(Thrombomodulin)的C端引入叠氮蛋氨酸,与mPEG2三芳基膦(mPEG2triarylphosphine)修饰剂生成稳定的单修饰物,尽管该方法比较复杂,但至少给我们提供了一个新的PEG修饰蛋白的思路
此类PEG定点修饰的原理是:首先用葡萄糖氧化酶或高磺酸钠氧化糖类残基产生具有反应活性的醛基,这些醛基可与mPEG2肼衍生物反应生成腙或与mPEG2胺衍生物反应生成可逆的Schiff碱。用硼氢甲腈钠可将腙还原为更稳定的烷基肼化物,而Schiff碱可以还原为仲胺。相对于mPEG-胺衍生物修饰,PEG-肼衍生物修饰不会形成交联聚合体,更适合于糖基的修饰,最有代表性的mPEG2肼衍生物是mPEG2酰肼(hydrazide2mPEG)。酰肼基团极低的pKa值使得酰肼基团在酸性环境下(pH4.5～5.0)中仍可以和羧基结合,而蛋白质中的氨基在该条件下由于发生质子化而不能与羧基反应,从而可实现选择性定位修饰。
由于蛋白种类多样和结构复杂,往往会使得PEG修饰反应特异性不强,反应条件要通过大量试验优化。如果能够利用计算机技术对蛋白质晶体结构表面性质进行理论计算和分析,确定可能的反应位点和基团,并根据被修饰基团与修饰剂的结构特点设计合适的连接物,这样不但可以减少PEG修饰的盲目性,而且能够提高研究效率。胡远东等利用InsightⅡ分子模拟软件包(BiosymInc.,SanDiego,CA)对牛血红蛋白晶体(bHb)进行了结构分析和分子模拟,计算了蛋白表面24个可进行PEG修饰的Lys残基及其主要原子的溶剂可及表面积,确定了其中可以进行修饰的13个Lys残基(溶剂可及表面积>0.4nm2),并进一步设计了具有双功能基团的小分子化合物环氧乙烷基活化PEG作为连接体。结果表明,PEG修饰的bHb的携氧能力和免疫原性等生物学指标均达到预期结果。
PEG衍生试剂材料的介绍：
西安瑞禧生物科技有限公司提供聚乙二醇衍生试剂其中PEG可修饰的基团包括以下：
PEG-OH 羟基
PEG-NHS 活化脂
PEG-SG 琥珀酰亚胺戊二酸酯 Succinimidyl Glutarate
PEG-MAL 马来酰亚胺
PEG-SH 巯基
PEG-Silane 硅烷
PEG-NH2 氨基
PEG-Biotin 生物素
PEG-SS 琥珀酰亚胺琥珀酸酯 Succinimidyl Succinate
PEG-GAS
PEG-ALD 丙醛
PEG-NPC 对硝基苯碳酸酯
PEG-OPSS 邻二硫吡啶
PEG-Hydrazide 酰井
PEG-Acrylate 甲氧基-聚乙二醇-丙烯酸酯
PEG-Azide 叠氮
PEG-Alkyne 炔基
PEG-CHO 醛基
PEG-DSPE 二硬脂酸磷脂酰乙醇胺
PEG-FITC 异硫氰酸荧光素
PEG-RB 罗丹明B
PEG-Chloride 氯化物
PEG-COOH 羧基
PEG-EPO
PEG-SVA 琥珀酰亚胺戊酸酯
PEG-SPA 琥珀酰亚胺丙酸酸酯
PEG-DBCO
PEG-LA 硫辛酸
PEG-胆固醇
PEG-Folic Acid 叶酸
mPEG L-Lysine 赖氨酸
mPEG Cysteine 半胱氨酸
mPEG Glutathione 谷胱甘肽
mPEG Aspartic Acid 天（门）冬氨酸
mPEG Glutamic Acid
Polylysine PEG
Dextran PEG
BSA PEG Conjugate
聚乙二醇修饰产品的详细产品列表介绍：
氨基产品目录表：
mPEG-NHS, MW:2000
mPEG-CHO, MW:350
mPEG-NPC, MW:550
mPEG-PFP, MW:1000
mPEG-S-S-NHS, MW:3400
mPEG-SPA, MW:5000
mPEG-SVA, MW:750
NHS-PEG-NHS, MW:10000
NHS-PEG-MAL, MW:5000
NHS-PEG-Biotin, MW:2000
NHS-PEG-FITC, MW:5000
NHS-PEG-Silane, MW:20000
Diethoxylsilane-PEG-NHS, MW:5000
Monoethoxylsilane-PEG-NHS, MW:5000
NHS-PEG-DSPE, MW:2000
NHS-PEG-CLS, MW:1000
NHS-PEG-COOH, MW:3400
NHS-PEG-OH
CHO-PEG-CHO, MW:550
NPC-PEG-NPC, MW:1000
mPEG-EPO, MW:20000
EPO-PEG-EPO, MW:1000
mPEG-ISC, MW:40000
mPEG-ITC. MW:2000
ISC-PEG-ISC, MW:3400
mPEG-Tosylate, MW:5000
巯基产品目录表：
Maleimide PEG, mPEG-MAL, MW:2000
Maleimide PEG Maleimide, MAL-PEG-MAL, MW:1000
Maleimide PEG NHS, MAL-PEG-NHS, MW:750
Maleimide PEG PFP, MAL-PEG-PFP, MW:3400
Maleimide PEG Biotin, MAL-PEG-Biotin, MW:5000
Maleimide PEG Fluorescein, MAL-PEG-FITC, MW:1000
Maleimide PEG Silane, MAL-PEG-Silane, MW:3400
Maleimide PEG Amine, MAL-PEG-NH2, MW:2000
Maleimide PEG Acid, MAL-PEG-COOH, MW:10000
Ortho-pyridine disulfide PEG, mPEG-OPSS, MW:750
OPSS-PEG-OPSS, MW:1000
OPSS-PEG-NHS, MW:2000
OPSS-PEG-Biotin, MW:2000
OPSS-PEG-NH2, MW:3400
OPSS-PEG-COOH, MW:550
Iodoacetyl PEG, mPEG-IA, MW:20000
Iodoacetyl PEG NHS, IA-PEG-NHS, MW:5000
Iodoacetyl PEG Maleimide, IA-PEG-MAL, MW:2000
Iodoacetyl PEG amine, IA-PEG-NH2, MW:3400
Iodoacetyl PEG Biotin, IA-PEG-Biotin, MW:1000
Iodoacetyl PEG Acid, IA-PEG-COOH, MW:5000
PEG di-iodoacetate, IA-PEG-IA, MW:3400
Vinylsulfone PEG, mPEG-VS, MW:2000
Vinylsulfone PEG acid, VS-PEG-COOH, MW:1000
Vinylsulfone PEG Fluorescein, VS-PEG-FITC, MW:5000
Vinylsulfone PEG Biotin, VS-PEG-Biotin, MW:20000
Vinylsulfone PEG DSPE, VS-PEG-DSPE, MW:550
Vinylsulfone PEG Silane, VS-PEG-Silane, MW:3400
Monofunctional PEGs产品目录表：
Methoxyl PEG, mPEG-OH
Amino PEG, Methoxy PEG Amine, mPEG-NH2
Carboxylic PEG acid, mPEG-COOH
Thiol PEG, methoxy PEG Thiol, mPEG-SH
Azido PEG, mPEG-N3
Hydrazide PEG, mPEG-HAZ
Acrylate PEG, mPEG-ACR
Succinimidyl PEG NHS, mPEG-NHS(SC)
Succinimidyl propionic acid PEG NHS, mPEG-SPA
Succinimidyl valeric acid PEG NHS, mPEG-SVA
Methoxyl PEG Aldehyde, mPEG-CH2CHO
PEG Nitrophenyl Carbonate PEG, mPEG-NPC
Epoxide PEG, mPEG-EPO
Isocyanate PEG, mPEG-ISC
Isothiocyanate PEG, mPEG-ITC
Maleimide PEG, mPEG-MAL
Ortho-pyridine disulfide PEG, mPEG-OPSS
Iodoacetyl PEG, mPEG-IA
Vinylsulfone PEG, mPEG-VS
PEG Biotin, mPEG-Biotin
Fluorescein PEG, mPEG-FITC
Rhodamine-PEG, mPEG-RB
Silane PEG, mPEG-Silane
Lipoic acid PEG, mPEG-LA
DSPE PEG, mPEG-DSPE
DMPE PEG, mPEG-DMPE
DPPE PEG, mPEG-DPPE
DLPE PEG, mPEG-DLPE
Cholesterol PEG, mPEG-CLS
DBCO PEG, mPEG-DBCO
Homobifunctional PEG
产品目录：
HO-PEG-OH
Amino PEG Amine, NH2-PEG-NH2
Thiol PEG Thiol, HS-PEG-HS
Carboxylic Acid PEG Acid, HOOC-PEG-COOH
Hydrazide-PEG-Hydrazide
Aldehyde PEG Aldehyde, CHO-PEG-CHO
Bifunctional NHS PEG, NHS-PEG-NHS
Bifunctional NPC PEG, NPC-PEG-NPC
Epoxide PEG Epoxide, EPO-PEG-EPO
Isocyanate PEG Isocyanate, ISC-PEG-ISC
Maleimide PEG Maleimide, MAL-PEG-MAL
OPSS-PEG-OPSS
PEG di-iodoacetate, IA-PEG-IA
Biotin-PEG-Biotin
PEG di-vinylsulfone, VS-PEG-VS
PEG di-acrylate, Acrylate-PEG-Acrylate
CDI-PEG-CDI
PFP-PEG-PFP, pentafluorophenyl PEG
Heterobifunctional PEG产品目录：
Amino PEG Acid, NH2-PEG-COOH
Amino PEG hydroxyl, NH2-PEG-OH
Maleimide PEG NHS, MAL-PEG-NHS
Maleimide PEG PFP, MAL-PEG-PFP
Maleimide PEG Amine, MAL-PEG-NH2
Maleimide PEG Acid, MAL-PEG-COOH
NHS-PEG-COOH
NHS-PEG-OH
Biotin-PEG-NHS
Biotin-PEG-NH2
Biotin-PEG-COOH
Biotin-PEG-Maleimide
Biotin-PEG-FITC
Biotin PEG Thiol, Biotin-PEG-SH
Thiol PEG Amine, HS-PEG-NH2
Thiol PEG Acid, HS-PEG-COOH
Thiol PEG OH, HS-PEG-OH
Thiol PEG Azide, HS-PEG-N3
Thiol PEG Fluorescein, HS-PEG-FITC
DSPE-PEG-NHS
DSPE-PEG-Maleimide, DSPE-PEG-MAL
DSPE PEG Biotin
DSPE PEG Amine, DSPE-PEG-NH2
DSPE PEG Acid, DSPE-PEG-COOH
Silane-PEG-Amine
Silane-PEG-COOH
Silane-PEG-Biotin
Silane-PEG-Maleimide
Silane-PEG-NHS
Silane-PEG-SH
Azido PEG hydroxyl, N3-PEG-OH
Azido PEG amine, N3-PEG-NH2
Azido PEG acid, N3-PEG-COOH
Azido PEG NHS, N3-PEG-NHS
Azido PEG Maleimide, N3-PEG-Mal
Azido PEG Biotin, N3-PEG-Biotin
Fluorescein PEG NHS, FITC-PEG-NHS
Fluorescein PEG Amine, FITC-PEG-NH2
Fluorescein PEG Maleimide, FITC-PEG-Mal
Fmoc-NH-PEG-NHS
Fmoc-NH-PEG-NH2
Fmoc-NH-PEG-COOH
Boc-NH-PEG-NHS
Boc-NH-PEG-NH2
Boc-NH-PEG-COOH
Acryloyl PEG COOH
Acryloyl PEG NHS
Acryloyl PEG NH2
Alkyne PEG amine, Alkyne-PEG-NH2
Alkyne PEG acid, Alkyne-PEG-COOH
Alkyne PEG thiol, Alkyne-PEG-SH
Alkyne PEG NHS, Alkyne-PEG-NHS
Alkyne PEG Maleimide, Alkyne-PEG-Mal
Alkyne PEG Biotin, Alkyne-PEG-Biotin
Biotinylated PEGs
Nanocs provides a variety of biotin PEG derivatives that include Biotin PEG NHS, biotin PEG maleimide, fluorescent PEG biotins, lipid PEG biotins and many other biotinylated,
multifunctional PEG reagents.
产品列表：
PEG Biotin, mPEG-Biotin
Biotin-PEG-NHS
Biotin-PEG-PFP
Biotin-PEG-NH2
Biotin PEG hydrazide
Biotin-PEG-COOH
Biotin-PEG-OH
Biotin-PEG-Maleimide
Biotin-PEG-FITC
Biotin-PEG-Biotin
Biotin-PEG-SH
Other Fluorescent PEG Biotin
Folic Acid PEG Biotin
Desthiobiotin-PEG-X
Iminobiotin-PEG-X
产品列表Surface Reactive PEGs：
Silane PEG, mPEG-Silane
Silane-PEG-Amine
Silane-PEG-COOH
Silane-PEG-Biotin
Silane-PEG-Maleimide
Silane-PEG-NHS
Silane-PEG-SH
Silane-PEG-OPSS
Silane-PEG-FITC
Silane-PEG-Acrylate
Silane-PEG-N3, Silane PEG Azide
Thiol PEG, methoxy PEG Thiol, mPEG-SH
Thiol PEG Amine, HS-PEG-NH2
Thiol PEG Acid, HS-PEG-COOH
Thiol PEG NHS, HS-PEG-NHS
Thiol PEG OH, HS-PEG-OH
Thiol PEG Biotin, HS-PEG-Biotin
Thiol PEG Azide, HS-PEG-N3
Thiol PEG Alkyne, HS-PEG-Alk
Thiol PEG Fluorescein, HS-PEG-FITC
Lipoic acid PEG, mPEG-LA
Lipoic acid PEG amine, LA-PEG-NH2
Lipoic acid PEG acid, LA-PEG-COOH
Lipoic acid PEG NHS, LA-PEG-NHS
Lipoic acid PEG, LA-PEG-Biotin
Lipoic acid PEG, LA-PEG-OH
Lipoic acid PEG, LA-PEG-FITC
Lipoic acid PEG, LA-PEG-NHS
Lipoic acid PEG, LA-PEG-MAL
Fluorescent PEGs产品目录表：
Fluorescein PEG, mPEG-FITC
Fluorescein PEG Amine, FITC-PEG-NH2
Fluorescein PEG hydroxyl, FITC-PEG-OH
Fluorescein PEG acid, FITC-PEG-COOH
Fluorescein PEG Thiol, FITC-PEG-SH
Fluorescein PEG Biotin, FITC-PEG-Biotin
Fluorescein PEG NHS, FITC-PEG-NHS
Fluorescein PEG Azide, FITC-PEG-N3
Fluorescein PEG Maleimide, FITC-PEG-Mal
Rhodamine-PEG, mPEG-RB
Rhodamine PEG hydroxyl, RB-PEG-OH
Rhodamine PEG Amine, RB-PEG-NH2
Rhodamine PEG Thiol, RB-PEG-SH
Rhodamine PEG Acid, RB-PEG-COOH
Rhodamine PEG Biotin, RB-PEG-Biotin
Rhodamine-PEG-NHS,RB-PEG-NHS
Rhodamine-PEG-MAL,RB-PEG-MAL
Cy5 PEG Amine, Cy5-PEG-NH2
Cy5 PEG NHS, Cy5-PEG-NHS
Cy5 PEG acid, Cy5-PEG-COOH
Cy5 PEG Maleimide, Cy5-PEG-Mal
Cy5 PEG Thiol, Cy5-PEG-SH
Cy5 PEG Biotin, CY5-PEG-Biotin
Cy5 PEG Folic acid, Cy5-PEG-FA
Cy3 PEG Amine, Cy3-PEG-NH2
Cy3 PEG NHS, Cy3-PEG-NHS
Cy3 PEG acid, Cy3-PEG-COOH
Cy3 PEG Maleimide, Cy3-PEG-Mal
Cy3 PEG Thiol, Cy3-PEG-SH
Cy3 PEG Biotin, Cy3-PEG-Biotin
Cy3 PEG Folic acid, Cy3-PEG-fa
Other Cy3 PEG Derivatives
Click PEG Derivatives产品目录表：
mPEG-DBCO
DBCO PEG NHS, DBCO-PEG-NHS
DBCO PEG Maleimide, DBCO-PEG-MAL
DBCO PEG Amine, DBCO-PEG-NH2
DBCO PEG acid, DBCO-PEG-COOH
DBCO PEG DSPE
DBCO PEG Silane
DBCO PEG Biotin
DBCO PEG Flurorescein, DBCO-EPG-FITC
DBCO PEG Cy3
DBCO PEG Cy5
Polymerizable PEGs产品目录表：
Acrylate PEG, mPEG-ACR
Methacrylate PEG, mPEG-MACR
Acrylate PEG NHS
Acrylate PEG Maleimide
Acrylate PEG Biotin
Acrylate PEG Amine, Acrylate-PEG-NH2
Acrylate PEG Acid, Acrylate-PEG-COOH
Acrylate PEG Acrylate
Multiarm PEGs产品目录表：
2 Arm/Branched PEG Acid 10000 Da
2 Arm/Branched PEG Acid 4000 Da
2 Arm/Branched PEG Amine 10000 Da
2 Arm/Branched PEG Amine 4000 Da
2 Arm/Branched PEG NHS 10000 Da
2 Arm/Branched PEG NHS 4000 Da
2 Arm/Branched PEG Maleimide 10000 Da
2 Arm/Branched PEG Maleimide 4000 Da
2 Arm/Branched PEG Biotin 10000 Da
2 Arm/Branched PEG Biotin
4-Arm PEG Acrylate
4-Arm PEG Amine
4-Arm PEG Thiol
4-Arm PEG Maleimide
4-Arm PEG NHS
4-Arm PEG Biotin
4 Arm PEG vinyl sulfone
8-Arm PEG Amine
8-Arm PEG Thiol
8-Arm PEG NHS
8-Arm PEG Maleimide
8-Arm PEG Biotin
8 Arm PEG vinyl sulfone
Other PEG Reagents产品目录表：
Folic Acid PEG, mPEG-FA Folic
Acid PEG Amine, FA-PEG-NH2
Folic Acid PEG Acid, FA-PEG-COOH
Folic Acid PEG Thiol, FA-PEG-SH
Folic Acid PEG DSPE
Folic Acid PEG NHS
Folic Acid PEG Maleimide
Folic Acid PEG Biotin
Folic Acid PEG FITC
Vitamin E PEG, mPEG alfa-tocopherol
Vitamin E PEG, mPEG alfa-tocopherol
Vitamin E PEG, mPEG alfa-tocopherol
Vitamin E PEG NHS, Tocopherol PEG NHS
Vitamin E PEG NHS, Tocopherol PEG NHS
Vitamin E PEG NHS, Tocopherol PEG NHS
Vitamin E PEG NH2, Tocopherol PEG amine
Vitamin E PEG NH2, Tocopherol PEG amine
Vitamin E PEG NH2, Tocopherol PEG amine
Vitamin E PEG Maleimide, Tocopherol PEG maleimide
Vitamin E PEG Maleimide, Tocopherol PEG maleimide
Vitamin E PEG Maleimide, Tocopherol PEG maleimide
Vitamin D PEG, mPEG Vitamin D
Vitamin D PEG, mPEG Vitamin D
Vitamin D PEG, mPEG Vitamin D
Vitamin D PEG, mPEG Vitamin D
Vitamin D PEG, mPEG Vitamin D
Vitamin D PEG NHS
Vitamin D PEG NHS
Vitamin D PEG NHS
Vitamin D PEG NH2
Vitamin D PEG NH2
Vitamin D PEG NH2
Vitamin D PEG Maleimide
Vitamin D PEG Maleimide
Vitamin D PEG Maleimide
Vitamin A/Retinoic acid PEG, mPEG Retinoic acid
Vitamin A/Retinoic acid PEG, mPEG Retinoic acid
Vitamin A/Retinoic acid PEG, mPEG Retinoic acid
Retinoic acid PEG NHS
Retinoic acid PEG Biotin
Retinoic acid PEG FITC
PEG amino acid/peptide Conjugates
产品列表：
mPEG L-Lysine
mPEG L-Lysine
Lysine PEG Lysine
4 Arm PEG L-Lysine
8 Arm PEG L-Lysine
mPEG Cysteine
Cysteine PEG Cysteine
4 Arm PEG Cysteine
mPEG Glutathione
Glutathione PEG Glutathione
4 Arm PEG Glutathione
mPEG Aspartic Acid
Aspartic Acid PEG Aspartic Acid
4 Arm PEG Aspartic Acid
mPEG Glutamic Acid
Glutamic Acid PEG Glutamic Acid
4 Arm PEG Glutamic Acid
PEG Metal Chelate Conjugates
mPEG DTPA
DTPA PEG Biotin
DTPA PEG FITC
4A PEG DTPA
mPEG NTA
NTA PEG Biotin
NTA PEG FITC
4A PEG NTA
NTA PEG Biotin
NTA PEG Biotin
BSA PEG Conjugate
Polylysine PEG Conjugate
Polylysine PEG Conjugate
Dextran PEG Conjugate
Dextran PEG Conjugate
Chymotrypsin PEG Conjugate
Superoxide Dimutase PEG
Lipase PEG
PEG esterase
Linear Monofunctional PEG Derivatives产品列表：
mPEG-OH , 1k/2k/5k/10k/20k/30k Da
mPEG-SCM , MW550/1k/2k/5k/10k/20k/30k Da
mPEG-SG , MW 550/1k/2k/5k/10k/20k/30k
mPEG-SS , MW 550/1k/2k/5k/10k/20k/30k
mPEG-GAS , MW 550/1k/2k/5k/10k/20k/30k
mPEG-SAS , MW 550/1k/2k/5k/10k/20k/30k
mPEG-ALD , 1k/2k/5k/10k/20k/30k
mPEG-Epoxide , MW 550/1k/2k/5k/10k/20k/30k
mPEG-NPC , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Silane , MW 550/1k/2k/5k/10k/20k/30k
mPEG-MAL , MW 550/1k/2k/5k/10k/20k/30k
mPEG-SH , MW 550/1k/2k/5k/10k/20k/30k
mPEG-OPSS , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Hydrazide , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Amine , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Acrylate , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Acrylamide , MW 550/1k/2k/5k/10k/20k/30k
mPEG-AA , MW 550/1k/2k/5k/10k/20k/30k
mPEG-GA , MW 550/1k/2k/5k/10k/20k/30k
mPEG-SA , MW 550/1k/2k/5k/10k/20k/30k
mPEG-GAA , MW 550/1k/2k/5k/10k/20k/30k
mPEG-SAA , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Azide , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Alkyne , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Biotin , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Dansyl , MW 550/1k/2k/5k/10k/20k/30k
mPEG-DSPE , MW 550/1k/2k/5k/10k/20k/30k
mPEG-DMPE , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Mesylate , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Tosylate , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Vinylsulfone , MW 550/1k/2k/5k/10k/20k
mPEG-Chloride , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Bromide , MW 550/1k/2k/5k/10k/20k/30k
mPEG-FITC , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Rhodamine , MW 550/1k/2k/5k/10k/20k/30k
mPEG-Cyanur , MW 550/1k/2k/5k/10k/20k/30k
Linear Homobifunctional PEG Derivatives 产品系列：
MAL-PEG-MAL , MW 600/1k/2k/3.4k/5k/10k/20k
OPSS-PEG-OPSS , MW 600/1k/2k/3.4k/5k/10k/20k
SH-PEG-SH , MW 600/1k/2k/3.4k/5k/10k/20k
SG-PEG-SG , MW 600/1k/2k/3.4k/5k/10k/20k
SS-PEG-SS , MW 600/1k/2k/3.4k/5k/10k/20k
GAS-PEG-GAS , MW 600/1k/2k/3.4k/5k/10k/20k
SAS-PEG-SAS , MW 600/1k/2k/3.4k/5k/10k/20k
Amine-PEG-Amine , MW 600/1k/2k/3.4k/5k/10k/20k
AC-PEG-AC , MW 600/1k/2k/3.4k/5k/10k/20k
ACA-PEG-ACA , MW 600/1k/2k/3.4k/5k/10k/20k
EP-PEG-EP , MW 600/1k/2k/3.4k/5k/10k/20k
NPC-PEG-NPC , MW 600/1k/2k/3.4k/5k/10k/20k
ALD-PEG-ALD , MW 600/1k/2k/3.4k/5k/10k/20k
AA-PEG-AA , MW 600/1k/2k/3.4k/5k/10k/20k
GA-PEG-GA , MW 600/1k/2k/3.4k/5k/10k/20k
SA-PEG-SA , MW 600/1k/2k/3.4k/5k/10k/20k
GAA-PEG-GAA , MW 600/1k/2k/3.4k/5k/10k/20k
SAA-PEG-SAA , MW 600/1k/2k/3.4k/5k/10k/20k
Azide-PEG-Azide , MW 600/1k/2k/3.4k/5k/10k/20k
Alkyne-PEG-Alkyne , MW 600/1k/2k/3.4k/5k/10k/20k
Biotin-PEG-Biotin , MW 600/1k/2k/3.4k/5k/10k/20k
Silane-PEG-Silane , MW 600/1k/2k/3.4k/5k/10k/20k
Hydrazide-PEG-Hydrazide , MW 600/1k/2k/3.4k/5k/10k/20k
Tosylate-PEG-Tosylate , MW 600/1k/2k/3.4k/5k/10k/20k
Chloride-PEG-Chloride , MW 600/1k/2k/3.4k/5k/10k/20k
Linear Heterobifunctional PEG Derivatives 产品列表：
HO-PEG-COOH , MW 1k/2k/3.4k/5k/10k
HO-PEG-NHS , MW 1k/2k/3.4k/5k/10k
HO-PEG-COOtBu , MW 1k/2k/3.4k/5k/10k
HO-PEG-NH2 , MW 1k/2k/3.4k/5k/10k
HO-PEG-NH-Boc , MW 1k/2k/3.4k/5k/10k
HO-PEG-NH-Fmoc , MW 1k/2k/3.4k/5k/10k
NH2-PEG-COOH , MW 1k/2k/3.4k/5k/10k
NH2-PEG-COOtBu , MW 1k/2k/3.4k/5k/10k
Boc-NH-PEG-COOH , MW 1k/2k/3.4k/5k/10k
Fmoc-NH-PEG-COOH , MW 1k/2k/3.4k/5k/10k
Boc-NH-PEG-SCM , MW 1k/2k/3.4k/5k/10k
Fmoc-NH-PEG-SCM , MW 1k/2k/3.4k/5k/10k
Boc-NH-PEG-NH2 , MW 1k/2k/3.4k/5k/10k
Fmoc-NH-PEG-NH2 , MW 1k/2k/3.4k/5k/10k
MAL-PEG-NH2 , MW 1k/2k/3.4k/5k/10k
MAL-PEG-OH , MW 1k/2k/3.4k/5k/10k
MAL-PEG-SCM , MW 1k/2k/3.4k/5k/10k
MAL-PEG-COOH , MW 1k/2k/3.4k/5k/10k
MAL-PEG-Silane , MW 1k/2k/3.4k/5k/10k
MAL-PEG-Acrylate , MW 1k/2k/3.4k/5k/10k
OPSS-PEG-NHS , MW 1k/2k/3.4k/5k/10k
OPSS-PEG-COOH , MW 1k/2k/3.4k/5k/10k
OPSS-PEG-OH , MW 1k/2k/3.4k/5k/10k
AC-PEG-SCM , MW 1k/2k/3.4k/5k/10k
AC-PEG-COOH , MW 1k/2k/3.4k/5k/10k
AC-PEG-NH2 , MW 1k/2k/3.4k/5k/10k
AC-PEG-NH-Boc , MW 1k/2k/3.4k/5k/10k
AC-PEG-OH , MW 1k/2k/3.4k/5k/10k
AC-PEG-Silane , MW 1k/2k/3.4k/5k/10k
Biotin-PEG-SCM , MW 1k/2k/3.4k/5k/10k
Biotin-PEG-MAL , MW 1k/2k/3.4k/5k/10k
Biotin-PEG-COOH , MW 1k/2k/3.4k/5k/10k
Biotin-PEG-NH2 , MW 1k/2k/3.4k/5k/10k
Biotin-PEG-OH , MW 1k/2k/3.4k/5k/10k
Biotin-PEG-Acrylate , MW 1k/2k/3.4k/5k/10k
Biotin-PEG-Silane , MW 1k/2k/3.4k/5k/10k
Thiol-PEG-Acid , MW 1k/2k/3.4k/5k/10k Da
Thiol-PEG-Amine , MW 1k/2k/3.4k/5k/10k Da
Thiol-PEG-OH , MW 1k/2k/3.4k/5k/10k Da
DSPE-PEG-MAL , MW 1k/2k/3.4k/5k
DSPE-PEG-COOH , MW 1k/2k/3.4k/5k
DSPE-PEG-NH2 , MW 1k/2k/3.4k/5k
DSPE-PEG-Biotin , MW 1k/2k/3.4k/5k
Multiarm PEG Derivatives – 4-Arm 产品列表：
4-Arm PEG-OH , MW 2k/5k/10k/20k
4-Arm PEG-Acrylate , MW 2k/5k/10k/20k
4-Arm PEG-Acrylamide , MW2k/5k/10k/20k
4-Arm PEG-Amine , MW 2k/5k/10k/20k
4-Arm PEG-Thiol , MW2k/5k/10k/20k
4-Arm PEG-MAL , MW2k/5k/10k/20k
4-Arm PEG-OPSS , MW2k/5k/10k/20k
4-Arm PEG-SCM, MW 2k/5k/10k/20k
4-Arm PEG-SG , MW 2k/5k/10k/20k
4-Arm PEG-SS , MW2k/5k/10k/20k
4-Arm PEG-GAS , MW 2k/5k/10k/20k
4-Arm PEG-SAS , MW 2k/5k/10k/20k
4-Arm PEG-Epoxide , MW 2k/5k/10k/20k
4-Arm PEG-NPC , MW 2k/5k/10k/20k
4-Arm PEG-Acid , MW 2k/5k/10k/20k
4-Arm PEG-GA , MW 2k/5k/10k/20k
4-Arm PEG-SA , MW 2k/5k/10k/20k
4-Arm PEG-GAA , MW 2k/5k/10k/20k
4-Arm PEG-SAA , MW 2k/5k/10k/20k
4-Arm PEG-Hydrazide , MW 2k/5k/10k/20k
4-Arm PEG-Azide , MW 2k/5k/10k/20k
4-Arm PEG-Alkyne , MW 2k/5k/10k/20k
4-Arm PEG-Biotin , MW 2k/5k/10k/20k
4-Arm PEG-Chloride , MW 2k/5k/10k/20k
4-Arm PEG-Tosylate , MW 2k/5k/10k/20k
Eight Arm PEG Derivatives 产品列表：
8-Arm PEG-OH , MW 10k/20k/40k
8-Arm PEG-Amine, MW 10k/20k/40k
8-Arm PEG-Acrylate , MW 10k/20k/40k
8-Arm PEG-Acrylamide , MW 10k/20k/40k
8-Arm PEG-Acid , MW 10k/20k/40k
8-Arm PEG-GA , MW 10k/20k/40k
8-Arm PEG-SA , MW 10k/20k/40k
8-Arm PEG-GAA , MW 10k/20k/40k
8-Arm PEG-SAA , MW 10k/20k/40k
8-Arm PEG-SCM , MW 10k/20k/40k
8-Arm PEG-SG , MW 10k/20k/40k
8-Arm PEG-SS , MW 10k/20k/40k
8-Arm PEG-GAS , MW 10k/20k/40k
8-Arm PEG-SAS , MW 10k/20k/40k
8-Arm PEG-Hydrazide , MW 10k/20k/40k
8-Arm PEG-Thiol , MW 10k/20k/40k
8-Arm PEG-MAL , MW 10k/20k/40k
8-Arm PEG-OPSS , MW 10k/20k/40k
8-Arm PEG-Epoxide , MW 10k/20k/40k
8-Arm PEG-NPC , MW 10k/20k/40k
8-Arm PEG-Azide , MW 10k/20k/40k
8-Arm PEG-Alkyne , MW 10k/20k/40k
8-Arm PEG-Biotin , MW 10k/20k/40k
8-Arm PEG-Chloride , MW 10k/20k/40k
8-Arm PEG-Tosylate , MW 10k/20k/40k
Lipid and Amphiphilic PEG Derivatives产品列表：
mPEG-O-C18 , PEG MW 550/1k/2k/5k Da
mPEG-CONH-C18 , PEGMW 550/1k/2k/5k Da
mPEG-O-C16 , PEGMW 550/1k/2k/5k Da
mPEG-CONH-C16 , PEGMW 550/1k/2k/5k Da
mPEG-O-C12 , PEGMW 550/1k/2k/5k Da
mPEG-CONH-C12 , PEGMW 550/1k/2k/5k Da
C18-PEG4-OH , PEG MW 176 Da
C18-PEG4-COOH , PEG MW 176 Da
C18-PEG4-NH2 , PEG MW 176 Da
C18-PEG4-Azide , PEG MW 176 Da
C18-PEG4-NHS , PEG MW 176 Da
C18-PEG4-MAL , PEG MW 176 Da
C18-PEG4-OPSS , PEG MW 176 Da
C18-PEG4-SH , PEG MW 176 Da
C18-PEG4-Hydrazide , PEG MW 176 Da
C18-PEG6-OH , PEG MW 264 Da
C18-PEG6-COOH , PEG MW 264 Da
C18-PEG6-NH2 , PEG MW 264 Da
C18-PEG6-Azide , PEG MW 264 Da
C18-PEG6-NHS , PEG MW 264 Da
C18-PEG6-MAL , PEG MW 264 Da
C18-PEG6-OPSS , PEG MW 264 Da
C18-PEG6-SH , PEG MW 264 Da
C18-PEG6-Hydrazide , PEG MW 264 Da
C18-PEG12-OH , PEG MW 528 Da
C18-PEG12-COOH , PEG MW 528 Da
C18-PEG12-NH2 , PEG MW 528 Da
C18-PEG12-Azide , PEG MW 528 Da
C18-PEG12-NHS , PEG MW 528 Da
C18-PEG12-MAL , PEG MW 528 Da
C18-PEG12-OPSS , PEG MW 528 Da
C18-PEG12-SH , PEG MW 528 Da
C18-PEG12-Hydrazide , PEG MW 528 Da
Polysaccharide Products - Hyaluronic Acid 产品列表：
Hyaluronic Acid , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Oligomeric-HA4 , MW 776.7 Da
Oligomeric-HA6 , MW 1156.0 Da
Oligomeric-HA8 , MW 1535.3 Da
Oligomeric-HA10 , MW 1914.6 Da
Hyaluronate-Biotin , MW 10k/20k/50k/250k/500k/750k/1000k/1500k/2500k Da
Hyaluronate-Mono-Biotin , MW 10k/20k/50k/250k/500k/1000k/1500k/2500k Da
Hyaluronate-Spacer-Biotin , MW 10k/20k/50k/250k/500k/750k/1000k/1500k/2500k Da
Biotin-Hyaluronate-Fluorescein , MW 10k/20k/50k/250k/500k/750k/1000k/1500k/2500k Da
Hyaluronate-Rhodamine , MW 10k/20k/50k/250k/500k/750k/1000k/1500k/2500k Da
Hyaluronate-Fluorescein , MW 10k/20k/50k/250k/500k/750k/1000k/1500k/2500k Da
Hyaluronate-AMCA , MW 20k/250k/500k/750k Da
Rhodamine-Hyaluronate-Fluorescein , MW 20k/500k/750k/1000k Da
Hyaluronic acid-NIR Dye , HA: 10k-2500k
Hyaluronate-Acrylate , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-Aldehyde , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-Amine , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-Epoxide , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-Maleimide , HA MW: 10k-2500k
Hyaluronate-NHS , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-Hydrazide , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-Thiol , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-Tyramine , MW 10k/50k/250k/750k/1000k/1500k/2500k Da
Hyaluronate-PEG , HA MW 10k/50k/250k/750k/1000k/1500k/2500k & PEG MW 2k
Hyaluronate-PEG , HA MW 10k/50k/250k/750k/1000k/1500k/2500k & PEG MW 5k
Hyaluronate-PEG , HA MW 10k/50k/250k/750k/1000k/1500k/2500k & PEG MW 10k
Ficoll, and Xylan 产品列表：
Alginate-Fluorescein, low viscosity 100-300 cP
Alginate-Fluorescein, medium viscosity 600-900 cP
Alginate-Fluorescein, high viscosity 1,000-1,500 cP
Alginate-Rhodamine, low viscosity 100-300 cP
Alginate-Rhodamine, medium viscosity 600-900 cP
Alginate-Rhodamine, high viscosity 1,000-1,500 cP
Alginate-Amine, low viscosity 100-300 cP
Alginate-Amine, medium viscosity 600-900 cP
Alginate-Amine, high viscosity 1,000-1,500 cP
Cellulose-Biotin , MW 90k/250k/720k/1300k Da
Cellulose-Fluorescein , MW 90k/250k/720k/1300k Da
Chondroitin-Sulfate , MW 25k/32k/40k/50k Da
Chondroitin-Sulfate-Biotin , MW 25k/32k/40k/50k Da
Chondroitin-Sulfate-Rhodamine , MW 10k/32k/40k/50k Da
Chondroitin-Sulfate-Fluorescein , MW 25k/32k/40k/50k Da
Chondroitin Sulfate-NIR Dye , CS MW: 25k-50k
Chitosan-Oligosaccharide , MW 2.5k/5k/7.5k Da
Chitosan , 10-50 cP, 85% DDA
Chitosan , 50-200 cP, 85% DDA
Chitosan , 200-500 cP, 85% DDA
Chitosan , 500-1000 cP, 85% DDA
Chitosan , 1000-3000 cP, 85% DDA
Chitosan , 10-50 cP, 95% DDA $200/5g
Chitosan , 50-200 cP, 95% DDA $200/5g
Chitosan , 200-500 cP, 95% DDA
Chitosan-Biotin , 10-50 cP, 85% DDA $250/10mg
Chitosan-Biotin , 200-500 cP, 85% DDA $250/10mg
Chitosan-Biotin , MW 200kDa, 95% DDA $250/10mg
Chitosan-Biotin , MW 200kDa, 85% DDA $250/10mg
Chitosan-Rhodamine , 10-50 cP, 85% DDA
Chitosan-Rhodamine , 200-500 cP, 85% DDA
Chitosan-Rhodamine , MW 200kDa, 95% DDA
Chitosan-Rhodamine , MW 200kDa, 85% DDA
Chitosan-Fluorescein , 10-50 cP, 85% DDA $250/10mg
Chitosan-Fluorescein , 200-500 cP, 85% DDA $250/10mg
Chitosan-Fluorescein , MW 200kDa, 95% DDA $250/10mg
Chitosan-Fluorescein , MW 200kDa, 85% DDA
Dextran-Biotin , MW 6k/20k/40k/70k/100k/550k/2000k Da
Dextran-Rhodamine , MW 6k/20k/40k/70k/100k/550k/2000k Da
Dextran-Fluorescein , MW 6k/20k/40k/70k/100k/550k/2000k Da
Dextran-NIR Dye , DE MW: 6k-2000k
Dextran-Amine , MW 6k/20k/40k/70k/100k/550k/2000k Da
CM-Dextran-Fluorescein , MW 4k/20k/40k/70k/150k Da
DEAE-Dextran-Fluorescein , MW 4k/20k/40k/70k/150k Da
Ficoll-Fluorescein , MW 20k/40k/70k/400k Da
Heparin-Biotin , MW 18 kDa
Heparin-Rhodamine , MW 18 kDa
Heparin-Fluorescein , MW 18 kDa
Heparin-NIR Dye , HP MW: 18kDa
Heparin-Amine , MW 18 kDa
Xylan-Fluorescein , MW 30 kDa
西安瑞禧生物科技有限公司的主营业务之一有PEG修饰试剂，代理美国nanocs和美国laysan的品牌，一直在国内销售着PEG种类最为齐全的产品，也是国内销售进口PEG最有影响力的公司之一，2014年由于公司业务发展需要，现推出自己的国产PEG品牌（Resenbio），我们自己品牌的PEG种类依旧很齐全，价格确比进口的产品低了很多。如果有需要了解的请与我们联系
西安瑞禧生物推出的国产PEG品牌（Resenbio）优势如下：
国产PEG种类最为齐全（高达三千种PEG修饰产品）
产品质量稳定可靠（纯度大于95%）
销售价格低廉（大部分产品价格是国外的50%甚至更低）
售后服务有保证（如果有任何质量问题可以退换货）
可提供各种复杂PEG合成定制服务
我公司推出自己的PEG品牌Resenbio PEG Book，我们提供国际范围内种类最为齐全的国产PEG产品，产品纯度高达95%，产品价格确比国外低了好多，如有需要可以咨询我们。
联系电话：029-86354885
销售经理：13309186942
销售邮箱：sales@xarxbio.com
sales@Resenbio.com
国内站：www.xarxbio.com
我们还提供特殊PEG产品的定制服务，联系邮箱如下：
特殊PEG定制邮箱：custservice@xarxbio.com
