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atilabiosystems 蛋白G琼脂糖

prootein G Agarose)现货85折促销

Protein A Agarose

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. It consists of a single polypeptide chain (MW 46,762) and contains little or no carbohydrate. Protein A binds specifically to the Fc region of immunoglobulin molecules, especially IgG. It has four high-affinity (Ka = 108/mole) binding sites that are capable of interacting with the Fc region of IgGs of several species (only two sites can bind at a time). The molecule is heat-stable and retains its native conformation even after exposure to denaturing reagents such as 4 M urea, 4 M thiocyanate and 6 M guanidine hydrochloride.

Highlights:

• Efficient and leach-resistant immobilization chemistries provide for high capacity and purity for antibody purification
• Binds to a wide range of antibodies - especially good for purification of rabbit IgG
• Available in regular and high binding-capacity formats
• Can be regenerated and reused multiple times when stored properly.

Specificity:

Best for polyclonal IgG from rabbit, pig, dog, cat serum; poor for Mouse IgG1, human IgG3, rat, goat, cow

Capacity ≥10 mg/mL binding capacity (rabbit IgG)

Physical form:  

Agarose (6%) Suspension in 0.5 M NaCl containing 0.02% thimerosal

Properties:

particle size: 45-165 μm (wet)

pore size:10,000-1,000,000 Da fractionation range (dextrans)

                10,000-4,000,000 Da fractionation range (globular proteins) 

 matrix activation: epoxy

 matrix attachment: ether

 matrix spacer: 16 atoms

Storage temperature: 2-8°C