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Thiol-reactive agarose

Thiol Reactive Agarose Resin

Features and Benefits 

Thiol-reactive agarose resins are porous, crosslinked 4% or 6% agarose beads that have been activated with bromoacetyl groups with a long flexible, hydrophilic triethyleneglycol linker. When incubated with a solution of thiol containing ligands including small molecules, oligonucleotides, peptides, and proteins that contains reduced cysteine residues, the bromoacetyl groups react specifically and efficiently with the exposed thiol groups (-SH) to form stable covalent thioether bonds that permanently attach the peptide or protein to the resin. Thus an affinity resin is made for purification of antibodies, antigens and other molecules of interest. Crosslinked and higher concentration (6%) of agarose provides more resistant to denaturing conditions, and thus offers more versatility in the choice of sample buffer and eluent.

Physical form:  

Suspension in 20% ethanol

Fucntional group: bromoacetyl

Linker length: 16 atoms, hydrophilic triethyleneglycol ether

Capacity: 10 to 15 micromoles bromoacetyl per ml gel

Properties:

particle size: 45-165 μm (wet)

pore size:10,000-1,000,000 Da fractionation range (dextrans)

                10,000-4,000,000 Da fractionation range (globular proteins) 

Storage temperature: 2-8°C, protected from extensive light.