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Twice as much signal for less than the GE ECL System
SuperSignal West Pico Chemiluminescent Substrate permits low picogram detection limits to be achieved when entry-level ECL substrates do not perform as desired. Using lower concentrations of antibody than standard ECL substrates, SuperSignal West Pico Chemiluminescent Substrate yields longer light emission and at least two times the signal intensity of other entry-level luminol-based horseradish peroxidase detection systems while maintaining sensitivity and low background. All of this for about two-thirds the price of comparable products.
SuperSignal West Pico Chemiluminescent Substrate is stable for at least one year at room temperature and can be stored at 4°C for longer stability. The working solution is stable for a minimum of 24 hours at room temperature and can be used in either light and dark conditions.
About SuperSignal West Pico Chemiluminescent Substrate
In side-by-side comparisons using identical conditions, blots incubated in SuperSignal West Pico Chemiluminescent Substrate exhibit at least twice the intensity of blots treated with the GE Healthcare ECL System. SuperSignal West Pico Chemiluminescent Substrate costs much less than the GE Healthcare Amersham ECL* System. Furthermore, the SuperSignal West Pico Substrate stock solution is room temperature (RT) stable for more than 12 months, with no discernable loss in activity. RT stability frees up valuable cold room space and saves time because there is no need to wait for the reagents to warm up. With signal duration of more than six hours, there is adequate time to optimize the exposure conditions. In most cases, there is no need to rerun gels. Switching to SuperSignal West Pico Substrate from an entry-level ECL substrate is not only easy, it will give you more of the results you are looking for.
Highlights for SuperSignal West Pico Chemiluminescent Substrate:
- Economy – costs less per milliliter than other similarly sensitive chemiluminescent substrates available
- Picogram sensitivity – highly sensitive for the rapid development of a wide range of protein levels
- Longer light emission – strong light emission over a working day allows you to make several exposures
- High intensity – signal is twice as intense as other luminol-based systems
- Excellent stability – 24-hour plus working solution stability; kit is stable for at least one year at ambient temperature
- Saves antibody – requires more dilute primary and secondary antibodies so you use less per blot
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Enhance light emission kinetics: Thermo Scientific SuperSignal Substrate vs. GE ECL System. Net relative intensity six hours after incubation is much greater for SuperSignal West Pico Substrate than for the GE ECL System.
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Thermo Scientific SuperSignal West Pico is more sensitive than GE ECL Substrate. Recombinant mouse IL-2 was diluted (50-0.003 ng) and electrophoresis was performed. The gels were transferred to nitrocellulose membranes, blocked and incubated with a 1 µg/ml dilution of rat anti-mouse IL-2. After washing, the membranes were incubated with 20 ng/ml dilutions of HRP-conjugated goat anti-rat antibody. The membranes were washed again and then incubated with substrate that was prepared according to the manufacturers' instructions. Blots were exposed to film for one- and five-minute exposures
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Related Products
Chemiluminescent Western Blot Substrate Selection Guide – Compare Pierce ECL and SuperSignal substrates to determine which substrate or Western blotting kit is best for your application.
Membranes, Filter Paper and Transfer Buffer
CL-XPosure Film
Related Links
Tech Tip # 67: Chemiluminescent Western blotting technical guide and protocols
Tech Tip # 21: Convert to SuperSignal West Pico Substrate from ECL Substrate
Tech Tip # 22: Determine source of nonspecific background signal in Western blots
Tech Tip # 24: Optimize antigen and antibody concentrations for Western blots
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