产品名称 : 碱性磷酸酶测试盒 | QuantiChrom™ Alkaline Phosphatase Assay Kit
分类名称 : 酶活性测定,血液尿液化学
产品介绍 : 测定方法 比色法（405nm）定量测定碱性磷酸酶活性。
产品说明 碱性磷酸酶（ALP）能够在碱性环境下催化水解磷酸酯，并生成一种有机根和无机磷酸盐。对于哺乳动物来说，这种酶主要存在于肝脏和骨骼中，ALP过高，可能与恶性胆管闭塞、原发胆汁性肝硬化、原发硬化性胆管炎和肝淋巴瘤等疾病有关。简单﹑直接﹑高度自动化的ALP活性检测手段是十分理想的。本公司研制的碱性磷酸酶检测试剂盒可直接对样本检测，免除了预处理过程。测试方法是利用磷酸对硝基苯酯能被ALP水解生成黄色产物的特性，在波长为405nm下检测，其颜色深浅程度与酶活性成正比。
产品特点 测量敏感、测量范围广：采用5μL血清或血浆样本，ALP活性检测范围在2-800 IU/L。操作简便：通过简单的“混合—测量”步骤，可以在5分钟内准确量化ALP的活性。高通量：所有试剂均可用于高通量液体处理系统。
产品用途 1. 可直接检测血清、血浆等样品中ALP的活性. 2. 可用于ALP产品的鉴定和质量检验。 3. 可用于评定和高通量筛选ALP的抑制剂。
试剂盒规格 50mL 缓冲液, 600μL pNPP溶液, 1.5 mL 醋酸镁, 10 mL 校准品.
储藏条件 试剂盒可在室温下运输，pNPP溶液在-20℃下保存，其他试剂在4℃下保存。保质期6个月.
DESCRIPTION Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. Our Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
KEY FEATURES High sensitivity and wide linear range. Use 5 μL serum or plasma sample. The detection limit is 2-800 IU/L. Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of ALP activity within 5 minutes. Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments.
APPLICATIONS Direct Assays: ALP activity in serum, plasma and other sources. Characterization and Quality Control for ALP production. Drug Discovery: high-throughput screen for ALP inhibitors and evaluation of ALP inhibitors.
KIT CONTENTS 50 mL Assay Buffer, 1.5 mL Mg Acetate, 600 μL pNPP Liquid, 10 mL Calibrator.
STORAGE CONDITIONS The kit is shipped at room temperature. Shelf life of at least 6 months.