产品名称 : α-淀粉酶测试盒 | QuantiChrom™ α-Amylase Assay Kit
分类名称 : 酶活性测定,血液尿液化学
产品介绍 : 测定方法 比色法（595nm）定量测定α-淀粉酶活性。
产品说明 淀粉酶属于糖苷水解酶一族，其作用于α- 1 ,4 -糖苷键将淀粉分解成葡萄糖分子。 α-淀粉酶（EC 3.2.1.1）可在淀粉链上的任意位置切割，最终产生麦芽三糖、麦芽糖、葡萄糖以及来自直链淀粉和支链淀粉的“短糊精”。α-淀粉酶是哺乳动物的一种主要消化酶。人体中淀粉酶水平的增加与唾液腺创伤，及唾液腺、胰腺发炎和肾功能衰竭引起的腮腺炎有关。简单、直接、自动化的α-淀粉酶活性检测手段十分令人期待。本公司的α-淀粉酶测试盒运用α-淀粉酶裂解一种不溶性蓝色直链淀粉成可溶性有色产物。在595nm处的吸光强度与样品中淀粉酶的活性成正比。
适用范围 直接检测血清、血浆、唾液、尿液和其它生物样品中α-淀粉酶的活性。
产品特点 灵敏、准确：96孔板线性检测范围2 - 300 U/L。 方便：仅需添加单一试剂5分钟后于室温或37°C下读取吸光度。
试剂盒组成(96孔板供100份检测) 20 mL 底物 (pH 7.0)， 10 mL 终止试剂， 2 mL (相当于 550 U/L) 校准液，
储存条件：将所有的试剂在2-8°C下贮存。保质期6个月。
Description Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure.Simple, direct and automation-ready procedures for measuring α-amylase activity are very desirable. our QuantiChromTM α-amylase Assay uses an insoluble dye-coupled substrate amylose azure, which is cleaved by α-amylase into soluble colored products. The color intensity, measured at 595 nm, is proportionate to the enzyme activity in the sample.
Applications Direct assays of α-amylase activity in serum, heparinized plasma, saliva, urine and other biological samples.
Key features Sensitive and accurate. Linear detection range 2 to 300 U/L α-amylase in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at 5 min at room temperature or 37°C.
Kit Contents 20 mL Substrate (pH 7.0), 10 mL Stop Reagent, 2 mL (equivalent to 550 U/L) Calibrator.
Storage conditions. Store all reagents at 2-8°C. Shelf life of at least 6 months (see expiry dates on labels).