| 货号: 26036 |
| 基因符号: RAS(G12D) |
| 描述: Anti-Ras(G12D) Mouse Monoclonal Antibody |
| 背景介绍: Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
| 免疫原: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin |
| 经过测试的应用: ELISA, WB, IF, IHC |
| 推荐稀释比: ELISA: 1: 100-1: 3000 WB: 1: 200-1: 1000 IF: 1: 50-1: 100 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| Host: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2a |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:  Western blot analysis of recombinant Ras WT and G12D proteins. Equal amounts of purified Ras WT (lane 1, Cat. # 10172) and G12D (lane 2, Cat. # 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody (Cat. # 26036) (Top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat. # 21021). 免疫荧光:  . HEK293T cells were transfected with pCDNA3-GFP-Ras(G12D) plasmid, pCDNA3-GFP-Ras(WT)plasmid,pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid,or pCDNA3-GFP-Ras(G12A)plasmid, then fixed and stained with Anti-Ras(G12D) monoclonal antibody (Cat. # 26036). |