| 货号: 26326 |
| 基因符号: CTCF; MRD21 |
| 描述: Anti-CTCF(R339W) Mouse Monoclonal Antibody |
| 背景介绍: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture. |
| 免疫原: A synthetic peptide from the internal region of CTCF which includes the mutation of R339W, human origin |
| 经过测试的应用: ELISA, WB, IHC |
推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 500-1: 1000
IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes R339W mutant, but not wild-type CTCF of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant CTCF (R339W) and wild-type proteins.Purified His-tagged CTCF (R339W) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with Anti-CTCF(R339W) monoclonal antibody (Cat. #26326). 免疫荧光:
HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (R339W) plasmid (right column), then fixed and stained with Anti-CTCF(R339W) monoclonal antibody (Cat. #26326). |