| 货号: 26137 |
| 规格: 100 μl |
| 基因符号: AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
| 描述: Anti-AKT1(E49K) Mouse Monoclonal Antibody |
| 背景介绍: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers. |
| 免疫原: A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin |
| 经过测试的应用: ELISA, WB, IHC |
推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 500-1: 2000
IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes E49K mutant, but not wild-type AKT1 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: 
Western blot analysis of recombinant AKT1(E49K) and wildtype proteins. Purified His-tagged AKT1(E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-AKT1(E49K) monoclonal antibody (Cat. #26137). |
免疫荧光: 
. HEK293T cells were transfected with pCDNA3-GFP-AKT1 (WT) plasmid (left column) or pCDNA3-GFP-AKT1(E49K) plasmid (right column), then fixed and stained with Anti-AKT1(E49K) monoclonal antibody (Cat. #26137). |