蛋白质印迹: 
Western blot analysis of recombinant EGFR(L858R) and wildtype proteins. Purified His-tagged EGFR(L858R) protein (amino acid 702-987, lane 2, Cat. # 10230) and corresponding wild type protein (amino acid 702-987, lane 1, Cat. # 10210) were blotted with anti EGFR(L858R) mouse monoclonal antibody (Cat. # 26082) . Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. # 28004). 免疫荧光: 
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR L858R) plasmid (right column), then fixed and stained with anti- EGFR(L858R) monoclonal antibody (Cat. # 26082). Immunohistochemistry: 
Immunohistochemical analysis of paraffin-embedded human NSCLC tissue with anti-EGFR(L858R) monoclonal antibody (Cat. #26082). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 400) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 100) was used as secondary antibody. |