| 货号: 26083 |
| 基因符号: EGFR |
| 描述: Anti-EGFR(G719S) Mouse Monoclonal Antibody |
| 背景介绍: EGFR is a receptor tyrosine kinase for ligands of the EGF family. EGFR activates several signaling cascades to convert extracellular cues into appropriate cellular responses. Defects in EGFR are associated with cancers such as lung cancer. |
| 免疫原: A synthetic peptide from the internal region of human EGFR, which includes the mutation of G719S |
| 经过测试的应用: ELISA, WB, IHC, IF |
推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 1000-1: 2000
IHC: 1: 50-1: 1000
IF: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2b |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes EGFR(G719S), but not wild type EGFR protein of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing. |
| 蛋白质印迹: 
Western blot analysis of recombinant EGFR(G719S) and wild-type EGFR proteins. Purified his-tagged EGFR (G719S) protein (aa 702-987) (Cat. #10214) and wild-type EGFR protein (aa 702-987) (Cat. #10210) were blotted with anti-EGFR(G719S) mouse antibody (Cat. #26083). 免疫荧光: 
HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (G719S) plasmid (right column), then fixed and stained with anti- EGFR (G719S) monoclonal antibody (Cat. #26083). |