| 货号: 26182 |
| 基因符号: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRaf(V600R) Mouse Monoclonal Antibody |
| 背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| 免疫原: A synthetic peptide from the internal region of BRaf(V600R), human origin |
| 经过测试的应用: ELISA, WB, IHC |
推荐稀释比:
ELISA 1: 1000-1: 5000
WB 1: 500-1: 1500
IHC 1: 100-1: 200 |
| 浓度: 0.9 mg/ml |
| 宿主: mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes B-Raf V600R, but not wild-type B-Raf proteins |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
 Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(V600R) protein ( lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf (V600R) monoclonal antibody (Cat. # 26182). |