| 货号: 26184 |
| 基因符号: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRaf(V600D) Mouse Monoclonal Antibody |
| 背景介绍: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| 免疫原: A synthetic peptide from the internal region of BRaf(V600D), human origin |
| 经过测试的应用: ELISA, WB, IF, IHC |
推荐稀释比:
ELISA 1: 1000-1: 5000
WB 1: 500-1: 2000
IF 1: 100-1: 200
IHC 1: 100-1: 200 |
| 浓度: 0.5 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Recognizes B-Raf V600D, but not wild-type B-Raf proteins from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: 
Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf(V600D) monoclonal antibody (Cat. # 26184). 免疫荧光: 
. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-BRaf(V600D) plasmid (right column), then fixed and stained with Anti-BRaf(V600D) monoclonal antibody (Cat. #26184). |