BRAF(V600A)小鼠单抗

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BRAF(V600A)小鼠单抗
产品简介
详细介绍
  • 参考报价：4800 产地：湖北 品牌：NewEast Biosciences 型号：BRAF(V600A) 更新时间：2023/3/20
货号:   26199
基因符号:   BRAF, BRAF1, RAFB1
描述:   Anti-BRAF(V600A) Mouse Monoclonal Antibody
背景介绍:   BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
免疫原:   A synthetic peptide from the internal region of BRAF, which includes the mutation of V600A, human origin
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1: 1000-1: 5000
WB: 1: 500-1: 1000
IF: 1: 50-1: 100
IHC: 1: 25-1: 100
浓度:   1 mg/ml
宿主:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯度:   Purified from ascites
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes V600A mutant, but not wild-type BRAF of vertebrates
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:   

Western blot analysis of recombinant BRAF proteins.

Purified His-tagged BRAF(V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF (V600R) protein (lane 3), His-tagged BRAF (V600M) protein (lane 4), His-tagged BRAF (V600K) protein (lane 5), His-tagged BRAF (V600G) protein (lane 6), His-tagged BRAF (V600D) protein (lane 7) and His-tagged BRAF (V600E) protein (lane 8) were blotted with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199).
免疫荧光:   

 HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid, pCDNA3-GFP-BRAF(V600A) plasmid, pCDNA3-GFP-BRAF (V600D) plasmid, pCDNA3-GFP-BRAF (V600G) plasmid pCDNA3-GFP-BRAF (V600M) plasmid or pCDNA3-GFP-BRAF (V600E) plasmid, then fixed and stained with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199).


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