| 货号: 26081 |
| 基因符号: IDH1 |
| 描述: Anti-IDH1(R132H) Mouse Monoclonal Antibody |
| 背景介绍: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| 免疫原: Recombinant protein of human IDH1 which includes the mutation of R132H |
| 经过测试的应用: ELISA, WB, IHC, IF |
推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 100-1: 1000
IF: 1: 50-1: 100
IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
| 蛋白质印迹: 
Western blot analysis of recombinant IDH1(R132H) and wild-type proteins. Purified His-tagged IDH1(R132H) protein (Cat.#10199) and wild-type protein (amino acid 1-240) (Cat.#10198) were blotted with anti-IDH1(R132H) mouse antibody (Cat.# 26081)(Left panel) or with anti-IDH1 mouse antibody (Cat. #26136 right panel). 免疫荧光: 
HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1(R132H) plasmid (right column), then fixed and stained with Anti-IDH1(R132H) monoclonal antibody (Cat. #26081). |