| 货号: 26406 |
| 基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| 描述: Anti-IDH1(R132P) Mouse Monoclonal Antibody |
| 背景介绍: Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| 免疫原: A synthetic peptide from the internal region of IDH1 which includes the mutation of R132P, human origin |
| 经过测试的应用: ELISA, WB, IF, IHC |
推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 100-1: 1000
IF: 1: 50-1: 100
IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes R132P mutant, but not wild-type IDH2 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: 
Western blot analysis of recombinant IDH1(R132P) and wildtype proteins.Purified His-tagged IDH1(R132P) (lane 1) and corresponding wild-type IDH1 protein (lane 2) were blotted with Anti-IDH1(R132P) monoclonal antibody (Cat. #26406). 免疫荧光:
HEK293T cells were transfected with pCDNA3-GFP-IDH1(R132P) plasmid, pCDNA3-GFP-IDH1 (WT) plasmid , pCDNA3-GFP-IDH1(R132S) plasmid, pCDNA3-GFP-IDH1(R132L) plasmid , pCDNA3-GFP-IDH1(R132G) plasmid or pCDNA3-GFP-IDH1(R132C) plasmid , then fixed and stained with Anti-IDH1(R132P) monoclonal antibody (Cat. #26406). |