| 货号: 26164 |
| 基因符号: IDH2(R172W) |
| 描述: Anti-IDH2(R172W) Mouse Monoclonal Antibody |
| 背景介绍: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172W IDH2 mutations do exist in diffusely infiltrative gliomas. |
| 免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172W, human origin |
| 经过测试的应用: ELISA, WB, IF, IHC |
推荐稀释比:
ELISA: 1: 1000-1: 5000
WB: 1: 100-1: 1000
IF: 1: 50-1: 100
IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes IDH1(R172W) of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
 Western blot analysis of recombinant IDH2(R172W) and wildtype proteins. Purified His-tagged IDH2(R172W) (lane 2) and wildtype protein (lane 1) were blotted with anti IDH2(R172W) monoclonal antibody (Cat. #26164). 免疫荧光: 
HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172W) plasmid (right column), then fixed and stained with anti-IDH2(R172W) monoclonal antibody (Cat. #26164). |