| 货号: 26084 |
| 基因符号: IDH2 |
| 描述: Anti-IDH2(R172H) Mouse Monoclonal Antibody |
| 背景介绍: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| 免疫原: A synthetic peptide from the internal region of human IDH2, which includes the mutation of R172H |
| 经过测试的应用: ELISA, WB, IHC, IF |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IF: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:  Western blot analysis of recombinant IDH2(R172H) and wild-type IDH2 proteins. Purified his-tagged IDH2(R172H) protein (Cat. # 10201) and wild-type IDH2 protein (Cat. # 10200) were blotted with anti-IDH2(R172H) monoclonal antibody (Cat. # 26084) (left panel). Proteins loading were blotted with anti-IDH2 monoclonal antibody (right panel). 免疫荧光:  HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172H) plasmid (right column), then fixed and stained with Anti-IDH2(R172H) monoclonal antibody (Cat. #26084). |