| 货号: 26908 |
| 规格: 30 μL |
| 基因符号: Gnaz |
| 描述: Anti-Gαz-GTP Mouse Monoclonal Antibody |
| 背景介绍: Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored. |
| 免疫原: Recombinant full length protein of active Gαz |
| 经过测试的应用: IP, IHC,IF |
推荐稀释比:
IP: 1 μg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-Gαz-GTP monoclonal antibody recognizes active Gαz of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹:
 Gαz activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-Gαz-GTP monoclonal antibody (Cat. No. 26908). Immunoblot was with an anti-Gαz polyclonal antibody. |