MidoriGreenAdvanceAgarose…

MidoriGreenAdvanceAgaroseTablets
产品简介
详细介绍
  • 参考报价:电议 产地:美国 品牌:Bulldog-Bio 型号:Midori Green 更新时间:2023/4/3

Don’t waste time pouring gels
Midori Green Advance Agarose Tablets are a fast, clean solution for preparing agarose gels without any additional time-consuming steps, such as weighing or adding different components. Just add the tablet to pure cold water, heat, and pour. That’s it! Once the gel hardens, it’s ready ready for loading. Each tablet contains the perfect amount of Midori Green Advance DNA Stain, with either no buffer, TAE powder or TBE powder, and 0.5g of the highest-purity agarose as integrated components; you don’t need anything else but water. If you’re tired of prepping agarose gels for your lab, this is the quickest and easiest solution to reduce effort and improve the quality of your gels.

Hey! The stain is already in that little tablet!
Another big advantage is safety. Midori Green Advance Nucleic Acid Stain is a new, safe alternative to traditional ethidium bromide(EtBr) stain for detecting dsDNA, ssDNA and RNA in agarose gels. Nearly identical to EtBr in performance and use, Midori Green is much less harmful to living organisms. As compared with EtBr, which is known as a strong mutagen, Midori Green causes many fewer mutations in the Ames test. When compared with spontaneous mutations caused by water in the Ames test, Midori Green’s rate was only slightly beyond the standard deviation range. In addition (and in contrast to EtBr), Midori Green scores negative in the mouse bone marrow micronucleus test and in the chromosome aberration test, which indicates that it’s considered non-carcinogenic. And for those who prefer to be safe rather than sorry, Midori Green has been tested for latex/nitrile glove penetration, in which both materials show negative results—even after 6 hours of exposure. Most importantly, Midori Green is not considered hazardous waste, and can be disposed of according to standard laboratory procedures.

Features:
– Tablet format – no weighing required
– Powder free
– Stable at room temperature when protected from light
– Only pure water is needed
– Fast dissolving protocol
– Non-carcinogenic alternative to ethidium bromide
– Environmentally safe – disposal with regular laboratory waste

 


Here you can see a gel which was produced with Midori Green Advance Agarose Tablets. 50 ml of water was added, creating a 1% agarose gel.

The steps could not be easier.
1) Add 1 tablet
2) Add 50 ml of water
3) Head up in a microwave until melted completely
4) Cool down the agarose to 60°C
5) Cast your gel


Safer for you and better for your subcloning
By using safe Midori Green dyes and safe Blue/Green LED illumination you can improve your subcloning transformation efficiencies by THREE-FOLD. In this-example, a plasmid vector was double digested with suitable restriction enzymes to create two sticky-ended DNA fragments: the lacZ gene (3,536 bp) and the backbone of the vector (4,318 bp). Equal amounts of digested DNA were electrophoresed on 1% agarose gels. The gels were stained with either ethidium bromide or Midori Green Direct gel stain according to the corresponding manuals, and then viewed using either a UV transilluminator or the FastGene Blue/Green LED Illuminator, respectively. The two DNA fragments were excised from the gels and purified using a silica membrane based purification kit. The lacZ gene and the vector backbone were religated using T4 DNA ligase transformed into DH5a cells and plated onto selection plates. The total number of blue and white colonies was counted to evaluate cloning efficiency. Each experiment was conducted in triplicate, and the average cloning efficiency was determined. Midori Green Direct resulted in dramatic increase of positive transformants.

Midori Green Can Boost Your Cloning Results!

Ethidium bromide is typically used in conjunction with a strong UV light source to excise DNA bands for purification prior to the ligation reaction. Short wave-length light is known to cause thymidine dimers and damage the DNA. The extent of this damage is not always appreciated. High energy light wreaks havoc on a DNA fragment in mere seconds. As can be seen below, after only a 15 sec exposure of DNA in a standard agarose gel, cloning efficiency starts to drop. And after a 30 sec exposure your cloning experiment is all but dead! In contrast, the cloning efficiency of protocols that use blue or blue/green LEDs are completely unaffected by this deleterious effect. If your lab isn’t able to break itself of its ethidium bromide habit, using a Blue/Green LED illuminator (or imaging system) should have an immediate positive impact on DNA integrity and cloning efficiency.


UV Transilluminators Kill Cloning Experiments

Just as sensitive as EtBr
Both dyes can stain single- and double-stranded nucleic acids with sensitivity and protocols similar to that of EtBr. This makes it a no-brainer as a drop-in replacement. Best yet, not only can you use your existing UV transilluminator and filter sets, Midori Green also works well when matched withBlue LED Gel Illuminators. These harmless light tables are the perfect complement for these stains, as you can work shield-less and goggle-less when excising bands or imaging migration. And because blue light does not cross-link DNA, there is never a worry about damaging DNA during cloning and photographing sessions.


不要浪费时间浇注凝胶
Midori Green Advance琼脂糖片剂是一种快速,清洁的解决方案,可用于制备琼脂糖凝胶,而无需任何其他费时的步骤,例如称重或添加不同的成分。只需将片剂加入纯净的冷水中,加热,然后倒入即可。而已!凝胶硬化后,就可以加载了。每片含有适量的  Midori Green Advance DNA染色剂,不含缓冲剂,TAE粉末或TBE粉末,以及0.5g最高纯度的琼脂糖作为集成成分;除了水,您不需要其他任何东西。如果您厌倦了为实验室准备琼脂糖凝胶,这是减少工作量并提高凝胶质量的最快,最简单的解决方案。

嘿! 污渍已经在那个小平板电脑中了!
另一个大优点是安全性。Midori Green Advance核酸染色剂是用于检测 琼脂糖凝胶中dsDNA,ssDNA和RNA的传统溴化乙锭(EtBr)染色剂的一种新型安全替代品Midori Green在性能和用途上与EtBr几乎相同,对生物的危害则小得多。与被称为强诱变剂的EtBr相比,Midori Green在Ames测试中引起的突变更少  与Ames测试中由水引起的自发突变相比,Midori Green的比率仅略微超出标准偏差范围。此外(与EtBr相比),绿豆绿在  小鼠骨髓微核试验  和 染色体畸变  测试,表明它被认为没有致癌性。对于喜欢安全而不是后悔的人,Midori Green经过了乳胶/丁腈手套渗透性测试,即使暴露6小时,两种材料仍显示阴性结果。最重要的是,Midori Green不被视为危险废物,可以按照标准实验室程序进行处置。

特点:
–片剂形式–无需称量–无
粉末
–避光保护在室温下稳定
–仅需纯水
–快速溶解方案
–溴化乙锭的非致癌替代物
–环保–定期处理实验室废物进行处置

 


在这里,您可以看到由Midori Green Advance琼脂糖片制成的凝胶。加入50ml水,产生1%琼脂糖凝胶。

步骤再简单不过了。
1)加1片
2)加50毫升水
3)用微波炉加热直至完全融化
4)将琼脂糖冷却至60°C 
5)浇铸凝胶


对您而言更安全,对亚克隆更好
通过使用安全的Midori Green染料和安全的Blue / Green LED照明,您可以通过三倍提高亚克隆转化效率。在该实施例中,质粒载体用合适的限制酶双重消化以产生两个粘性末端DNA片段:lacZ基因(3,536bp)和载体主链(4,318bp)。在1%琼脂糖凝胶上电泳等量的消化DNA。根据相应的手册,将凝胶用溴化乙锭或Midori Green Direct凝胶染料染色,然后分别使用UV透射仪或FastGene蓝/绿LED照射仪进行观察。从凝胶上切下两个DNA片段,并使用基于二氧化硅膜的纯化试剂盒纯化。使用转化到DH5a细胞中的T4 DNA连接酶将lacZ基因和载体主链重新连接,并铺在选择板上。计算蓝色和白色菌落的总数以评估克隆效率。每个实验一式三份进行,并确定平均克隆效率。Midori Green Direct导致阳性转化体的急剧增加。

Midori Green可以提高您的克隆结果!

溴化乙锭通常与强紫外线光源结合使用,以切除DNA条带,以便在连接反应之前进行纯化。短波长的光会引起胸苷二聚体并破坏DNA。这种损坏的程度并不总是可以理解的。高能光在短短几秒钟内对DNA片段造成破坏。如下所示,在标准琼脂糖凝胶中仅暴露DNA 15秒后,克隆效率开始下降。经过30秒的曝光后,您的克隆实验几乎已死!相反,使用蓝色或蓝色/绿色LED的协议的克隆效率完全不受此有害影响的影响。如果您的实验室无法摆脱自己的溴化乙锭习惯,请使用蓝色/绿色LED照明器 (或成像系统)应立即对DNA完整性和克隆效率产生积极影响。


紫外线透射仪杀死克隆实验

与EtBr一样敏感
两种染料都可以用与EtBr相似的灵敏度和方法对单链和双链核酸进行染色。这使它成为了直接的替代品。最好的是,不仅可以使用现有的紫外线透射照明器和滤光片组,而且与蓝色LED凝胶照明器搭配使用时,绿绿也可以很好地工作这些无害的光桌是这些污渍的完美补充,因为在切割乐队或成像迁移时,您可以无遮罩和无护目镜地工作。而且,由于蓝光不会使DNA交联,因此不必担心在克隆和照相过程中会损坏DNA。

 1 kb DNA阶梯经Midori Green 
和EtBr 染色
用Midori Green和EtBr染色的100bp DNA阶梯



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