Radnoti隔离血管灌注系统设计用于允许研究人员在温度受控的环境中测量血管段的收缩反应。血管或其他管状肌肉结构（例如小肠段）安装在穿过浴帽的一对玻璃套管上。每套有一个笔直的套管和一个J形套管；血管连接到J形的末端并连接到笔直的套管，从而形成一个环，血管可以通过该环进行灌注。通过以设定的速率灌注血管，可以通过在线压力传感器记录的压力变化来检测血管直径的变化。安装套管的位置可通过使用浴帽中内置的螺丝锁定位套管来适应容器的长度，将血管末端插入每个插管的末端，并将血管绑到位。盖子还具有位于血管两侧的铂场电极，可对肌肉进行电刺激。然后将带有容器的盖子插入温度控制槽中。
浴槽具有允许连续或作为离散洗涤液交换浴液的入口。因此，如果需要，可以向容器内部和外部灌注相似或不同的流体（即使在不同的温度下），并且可以连续收集流体。流体也可以独立充气，使研究人员可以创建不同的内部和外部有氧环境
Radnoti Glass Technology开发了一种特殊的灌注系统，以满足对内皮功能，内源性神经递质和血管活性物质的代谢和释放迅速发展的血管研究感兴趣的科学家的需求。
该系统包括一个Radnoti 25毫升器官浴，该浴池的水套中装有灌注液预热盘管。溶液通过玻璃鲁尔接头流出，然后通过连接管进入入口套管。在预热线圈的入口和出口使用Luer配件可使该系统与另一个灌注系统相连，从而形成一个级联系统（请参见方框）。
这种配置还允许放置压力传感器，并允许在灌注介质到达血管之前添加或移除灌注介质。灌注系统还具有带可调节针阀的可更换曝气器，底部排水孔和溢流出口。灌注系统盖由高密度聚丙烯制成，并带有O形圈密封以确保紧密贴合。盖子上有五个孔，用于穿过一组刺激电极以及一个流入和流出套管（在其间安装有血管）和一个用于将样本添加到浴槽内部的管。插入盖中的所有物品都可以使用尼龙紧定螺钉定位。
为了使用该灌注系统，将选择的血管解剖并清洗。然后通过缝合将血管固定到流入和流出套管上。流入套管是直的，而流出套管的一端是钩状的，以利于正确对准血管。插管的两端均略微张开，并具有精细的研磨表面，以确保与血管的牢固配合。
标准插管的外径为1.0、1.5、2.0、2.5和3.0mm，可容纳各种制剂，例如大鼠尾动脉，门静脉，肺动脉和肠系膜或孤立的大血管。可根据要求提供其他尺寸。该设计采用了开放式灌注系统，用于研究血管收缩剂和血管扩张剂，神经刺激以及各种药物在体外对整个血管床的作用。
刺激电极位于组织中，以促进神经递质的释放。当安装组织时，将电极向上滑动并用固定螺钉固定，从而可以清楚地进入套管。一旦组织被固定，电极就被重新定位。可以通过使用底部排水孔或溢流出口以及通过移液器，注射器或外部容器添加的溶液来快速更换浴液。
用一组插管中的一个插管该容器部分，并将其置于可充气的水套浴中。在恒压模式下，将储液罐（或气泡收集器）放在给定静水压头的熔池上方，并通过容器的收缩或膨胀来改变流量。流量测量是使用降落计数器，流量计或其他设备进行的。在恒定流量模式下，蠕动泵或注射泵以预定速率输送流体。在线压力传感器通过压力变化测量直径变化。
在恒定流量系统中，可以通过注射泵将药物注入系统中。在两种模式下，均可独立于通过血管灌注的介质更换血管外部的介质。也可以使用摄像机直接测量容器的直径。请注意，在某些情况下，可以使用水族馆泵为非碳酸氢盐缓冲系统中的氧气需求量低的组织充气，从而消除了气罐和调节器的问题和费用。
四通道系统中包含的仪器包括：4个压力传感器，蠕动泵，储液器，气泡收集器，循环浴以及所有相关的管道和连接器。单通道系统不包括泵或循环浴。可用选项：用于组织刺激的留置电极，组织刺激器，流量计或其他流体测量设备，照相机，气泵以及自动化的数据采集。
血管灌注系统有两种尺寸：
158700-1单通道血管灌注系统
158700-4四通道血管灌注系统
The Radnoti isolated blood vessel perfusion system is designed to permit the researcher to measure the contractile responses of blood vessel segments in a temperature controlled environment. Blood vessels, or other tubular muscular structures such as small sections of intestine, are mounted on a pair of glass cannula that pass through the cap of the bath. There is one straight cannula and one J-shaped cannula per set; the vessel is attached over the end of the J-shape and connected to the straight cannula, thus forming a loop through which the vessel can be perfused. By perfusing the vessel at a set rate, changes in vessel diameter can be detected by pressure changes registered by in-line pressure transducers. The mounting cannula are set in position to accommodate the vessel’s length by positioning the cannula using the screw locks built into the cap of the bath, inserting the ends of the vessel over the end of each cannula and tying the vessel onto position. The cap also has platinum field electrodes positioned on either side of the vessel, permitting electrical stimulation of the muscle. The cap with attached vessel is then inserted into the temperature controlled bath.
The bath has access ports that permit exchange of the bath fluid, either continuously or as discrete washes. Therefore, the vessel can be perfused internally and externally with similar or different fluids (even at different temperatures) and the fluids can be collected on a continual basis, if desired. The fluids can also be gassed independently, allowing the researcher to create different internal and external aerobic environments
Radnoti Glass Technology has developed a special perfusion system to meet the needs of scientists interested in blood vessel research in the rapidly advancing areas of endothelial function, metabolism and release of endogenous neurotransmitters and vasoactive substances.
This system includes a Radnoti 25ml organ bath, which has a perfusate pre-warming coil built into the bath’s water-jacket. The solution exits through a glass Luer fitting before passing into the entry cannula through connecting tubing. The use of Luer fittings on the entry and exit of the pre-warming coil permit the system to be coupled to another perfusion system to form a cascade system (see Box).
This configuration also allows placement of a pressure transducer and permits addition or removal of perfusion media before it reaches the blood vessel. The perfusion system also has a replaceable aerator with adjustable needle valve, a bottom drain and overflow outlet. The perfusion system cap is made of high-density polypropylene with an O-ring seal to insure a snug fit. The cap has five holes drilled through it for passage of a set of stimulating electrodes and an inflow and outflow cannula, between which the blood vessel is mounted, and a tube for sample addition to the bath interior. All of the items inserted into the cap can be positioned through the use of nylon set screws.
To use this perfusion system, the blood vessel of choice is dissected out and cleaned. The vessel is then secured to the inflow and the outflow cannula by means of a suture. The inflow cannula is straight while the outflow has a hooked end to facilitate proper vessel alignment. The ends of the cannula are both slightly flared with a fine ground finish to insure a secure fit to the blood vessel.
Standard cannula O.D.’s are 1.0, 1.5, 2.0, 2.5 and 3.0mm which will accommodate various preparations such as rat tail artery, portal vein, pulmonary aorta, and the mesentery or isolated large vessels. Additional sizes are available upon request. This design incorporates an open-end perfusion system for the study of vasoconstrictors and vasodilators, nerve stimulation, and the effect of various drugs on an entire vascular bed in-vitro.
Stimulating electrodes are positioned in the tissue to promote the release of neurotransmitters. When mounting the tissue, the electrodes are slid upward and secured with set screws, providing clear access to the cannula. Once the tissue is secured, the electrodes are repositioned. The bath media can be rapidly exchanged through the use of the bottom drain or the overflow outlet and solutions added via pipette, syringe or external reservoir.
The vessel section is cannulated with one of a set of cannula and placed in a water-jacketed bath that can be aerated. In the constant pressure mode, a reservoir (or bubble trap) is placed above the bath at a given hydrostatic head and flow is changed by the contraction or dilation of the vessel. Flow measurements are made using drop counters, flow meters or other devices. In the constant flow mode, a peristaltic or syringe pump delivers fluid at a predetermined rate. In-line pressure transducers measure changes in diameter via changes in pressure.
In the constant flow system, drugs may be infused into the system via syringe pumps. In both modes, the media outside the vessels can be changed independently of the media perfused through the vessels. It is also possible to directly measure the diameter of the vessel using a camera. Note that in certain cases an aquarium pump can be used to aerate tissues with low oxygen requirements in non-bicarbonate buffer systems, thereby eliminating the problems and expense of gas tanks and regulators.
Instrumentation included in the Four Channel system includes: 4 pressure transducers, a peristaltic pump, reservoirs, bubble traps, recirculating bath, and all associated tubing and connectors. The Single Channel system does not include pumps, or recirculating bath. Available options: Indwelling electrodes for tissue stimulation, a tissue stimulator, flow meters, or other fluid measuring devices, camera, air pump, and data acquisition with automation.
The Blood Vessel Perfusion System is available in two sizes:
158700-1 Single Channel Blood Vessel Perfusion System
158700-4 Four Channel Blood Vessel Perfusion System