bpsbioscience，50222，BCL-2TR-FRETAssayKit

Description:

The BCL-2 TR-FRET Assay Kit is designed to measure the inhibition of BCL-2 binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbiumlabeled donor, dye-labeled acceptor, BCL-2, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Synonym(s):

Bcl, Bcl2, B cell lymphoma 2, HTRF, BCLXL, BCL2

Format:

COMPONENTS:

Instructions for use:

See assay kit data sheet for detailed protocol.

Storage / Stability:

At least 6 months from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Reference(s):

Filippakopoulos, P., et al.,Cell 2012; 149:214.

Notes:

Since January of 2018, this kit has been optimized for better performance. The previous version of the BCL-2 TR-FRET Assay Kit, Cat. #50222, can still be purchased upon special request.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Apoptosis

Product Type:

Assay Kit

bpsbioscience，50222，BCL…